{"date" : "2013-07-28","author" : "fabio","title" : " the blue ligation: the never ending story !","content" : " today I started with the fourth ligation and then I transformed for the fourth time.Meanwhile I minipreppeed inocula from the second ligation, but screening wasn’t successful!!I also inoculated 6 colonies from the third ligation plates( only the plate 1:1).","tags" : " YF1_FixJ - FixK2"}