"

From 2013.igem.org

{"date" : "2013-07-29","author" : "emil","title" : "The incompetent Bacillus saga:the final transformation","content" : "Finally we have decided to try to transform PXyl+GFP that is an integrative vector for the thr locus.I have digested 1µg PXyl with the enzyme Sca1 following the standard protocol for the double digestion and exploiting the specific buffer included in the kit from Biolabs.I have previously grown B. subtilis in the medium from Groeningen o.n.,this morning I diluted the Bacillus(1:100) in the same medium then when it reached the O.D. of 1.1 I added 1µl of DNA and I let grow for two hours.After that I plated on spectinomicin added LB agar(stock solution 100mg/ml).","tags" : "B.subtilis-PXyl"}