{"date" : "2013-07-30","author" : "fabio","title" : " the blue ligation: the never ending story !","content" : " today unfortunately I miniprepped the last inocula (from ligation nr. 4) and screening didn’t verify the presence of any ligation. What a drag!! The only thing that remains to be done is to inoculate more and more colonies from previous plates: 6 from ligation 3 (plate 1:1) and 6 from ligation nr. 4 (plate 1:1).","tags" : " YF1_FixJ - FixK2"}