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From 2013.igem.org

{ "date" : "2013-08-04", "author" : "caterina", "title" : "27 minipreps, 27 fails", "content" : "Today it was an hard day, after 27 miniprep and 27 screening I didn't obtain positive results, so I restarted the digestion, ligation and trasformation of R0010 + K592009 in pSB1C3 and in pSB1A2.", "tags" : "light receptor" }