Team:UNITN-Trento/Notebook/Labposts/08/20

From 2013.igem.org

{ "date" : "2013-08-07", "author" : "emil", "title" : "Test for integration in B. subtilis ", "content" : "In order to demonstrate the integration of pXyl in Thr locus (essential for the biosinthesis of threonine) I have prepared 40 ml of a minimal medium (MNGE) following the LMU munich protocol without the threonine.Then I did an inoculum from the plate obtained from the transformation of 29/7.

MNGE medium
CompoundAmount
10xMN-Medium3,68ml
Sterile water33,12ml
Glucose (20%)4ml
K-Glutamat (40%)200µl
Fe[III]-ammonium-citrate (2,2 mg/ml)200µl
Tryptophan (5 mg/ml)400µl
MgSO4 (1M)120µl
threonine (5 mg/ml)(in this case absent)400µl
MN medium 10x 50 ml
CompoundAmount
K2HPO4 (x3 H2O)6,8 g
KH2PO43 g
Na-citrate (x 2 H2O) 0.5 g
", "tags" : "B.subtilis-PXyl" }