{ "date" : "2013-08-16", "author" : "emil", "title" : " To be continued", "content" : " I added 1 µl of DPN1 and SAP respectively to the insert and to the backbone, after 2 hours I inactivated the enzymes by heating for 20 minutes at 80 °. Afterwards I purified the samples following the purification protocol .Then I performed the ligation of the 2 parts following the ligation protocol . Finally I transformed the entire ligation in E. coli.", "tags" : "pXyl-GFP" }