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From 2013.igem.org

{ "date" : "2013-09-10", "author" : "gabriele", "title" : "A long day", "content" : "I prepared competent NEB10β cells with the usual protocol. I miniprepped mutB inocula and prepared inocula of pLac+SAMsynthetase and Wintergreen+pLac+SAMsynthetase. I plated the new competent cells on CM to detect any contamination. I performed a PCR to extract EFE from pucEFE3 (buffer HF).I also purified Viola's EFE PCRs and digested EFE (PCR insert, XbaI and PstI) and mutB (SpeI and PstI) overnight.", "tags" : "EFE-BlueLight" }