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From 2013.igem.org

{ "date" : "2013-09-11", "author" : "gabriele", "title" : "Cloning", "content" : "I treated the o/n digestion of mutB with SAP and of EFE with DpnI. I miniprepped yesterday inocula with bad results (I used a new kit) and screened EFE PCR, which was positive!

Then I ligated the purified O/N digestions: Ctrl, 1:1 and 1:2. I transformed the ligation in NEB10β and plated them.", "tags" : "EFE-BlueLight" }