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Team:UT Dallas/calender
From 2013.igem.org
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Calendar
7/5/2013
Transformation
[GFP] Kit plate 5. Well 14k. Backbone pSB1A2. Resistance A. i5-5-1
[LacI] Kit plate 5. Well 20. Backbone pSB1A2. Resistance A. i5-5-2
7/6/2013
Colony Picking
I5-5-1 → 2 colonies → picked 2 → i5-5-3
→ i5-5-4
I5-5-2 → 0 colonies
7/7/2013
Mini Prep
I5-5-3: 67.4 ng/ul [GFP]
I5-5-4: 98.7 ng/ul [GFP]
I5-5-5: 408.7 ng/ul [+ confiol]
Test Digestion
<a href="#" name="d2fd616f7186be08d9c7705395c2136d5ae85998"></a><a href="#" name="0"></a>I5-5-31 | I5-5-42 | |
DNA | 4 | 2 |
BSA | 1 | 1 |
Buffer 4 | 1 | 1 |
EcoRI | 1 | 1 |
Pst | 1 | 1 |
H2O | 2 | 4 |
10 | 10 |
