Team:UT Dallas/week6

From 2013.igem.org

8/11

<a name="h.vvmiq3irzx74"></a>picking colonies from transformation 8/10

<a name="h.xcc9g4d1gada"></a>

<a name="h.olbwgp15g6t9"></a>i5-30-3 → i5-30-8

<a name="h.21q6gds1ehex"></a>        i5-30-9

<a name="h.bzlm1jlyjh80"></a>

<a name="h.5wc3l82d0jxe"></a>Inoculate from glycerol stock:

<a name="h.4vfyb030afzi"></a>i5-22-5 [Scr]

<a name="h.t60w4w1qz78e"></a>i5-11-5 [pLac-GFP]

<a name="h.jmmhjdd89zr4"></a>

<a name="h.sthj625sbh1j"></a>1 → i5-11-5        61.1

<a name="h.9r999ot36cnq"></a>2 → i5-11-5        70.5

<a name="h.32qb54z130re"></a>3 → i5-11-5        58.6

<a name="h.db6xyee79l2m"></a>4 → i5-11-5        53.2

<a name="h.6eijjl1so9ik"></a>5 → i5-11-5         89.5

<a name="h.hgiet1dz05mf"></a>

<a name="h.5dtixuf80mfh"></a>Digestion

<a name="h.i3wavv6tqk2b"></a>

<a href="#" name="c3a2081ed35a86c2714ec99c9835d13d47592d1a"></a><a href="#" name="39"></a><tbody></tbody>

<a name="h.8vt5g222tbak"></a>

<a name="h.riiwupqmqcoi"></a>i5-31-1

<a name="h.jsoa1kpxc4vv"></a>i5-31-2

<a name="h.5ruk5040g5t4"></a>i5-31-3

<a name="h.u0empn399lsg"></a>i5-31-4/5

<a name="h.hnfez9nn2o8k"></a>Dna

<a name="h.22wlwhnoii02"></a>ComA (61.1) 32.7

<a name="h.ivc0psafpm3j"></a>ComD (70) 28.5

<a name="h.duls23yq14bd"></a>ComE (58.6) 34.1

<a name="h.pr2gmd48fifd"></a>Dex 30

<a name="h.fcegagyihx5l"></a>water

<a name="h.h60bu4sdaasu"></a>11

<a name="h.nfgfyiefc5up"></a>15

<a name="h.be22s9c5w1j6"></a>10

<a name="h.lecx4rtgqph2"></a>13

<a name="h.sw6apjw78neq"></a>Buffer

<a name="h.mhn3h9cykay"></a>5

<a name="h.3cyhgka656mz"></a>5

<a name="h.7ys3s1iv9zj8"></a>5

<a name="h.4d9gi6dnihxw"></a>5

<a name="h.vxbk98y03h1i"></a>EcoR

<a name="h.vj3akwf13l2h"></a>1

<a name="h.z6mcgyoplpso"></a>0

<a name="h.k133bsa79gki"></a>0

<a name="h.jqxb8sunh8wq"></a>0

<a name="h.kkvqfofng39p"></a>PstI

<a name="h.w6m4vgeqx9h5"></a>0

<a name="h.rd9ij47q4kjx"></a>0

<a name="h.dw7g5lxl7xhz"></a>1

<a name="h.bm7ymci4yk4a"></a>0

<a name="h.5tckkbw5ia12"></a>XbaI

<a name="h.bc4tflx1zoj8"></a>0

<a name="h.scgko0f8q9w7"></a>1

<a name="h.o13kh3yf1av4"></a>1

<a name="h.tbrncavf235g"></a>1

<a name="h.5uedgdiksfu6"></a>SpcI

<a name="h.n2xj9c6hsus1"></a>1

<a name="h.k6jj4syfvv0l"></a>1

<a name="h.swtckdt4933u"></a>0

<a name="h.92mfu5ky8kx8"></a>1

<a name="h.2y54s12xhlbx"></a>

<a name="h.ugixtn8vvmug"></a>i5-31-1                16.5

<a name="h.7wuuci41molh"></a>i5-31-2                10.3

<a name="h.nfcfce2p4r66"></a>i5-31-3                21.0

<a name="h.1lia2mtp2vk9"></a>i5-31-5                12.1

<a name="h.tidyk8jqpoz9"></a>

<a name="h.c8mms58srvew"></a>8/12

<a name="h.z60iu2l2d4j"></a>Made glycerol stock of:

<a name="h.si7nyr3khs9e"></a>i5-30-1 [pFru-RBS-GFP]

<a name="h.dfxrx1h9mii1"></a>i5-30-8 [pScr-RBS-GFP]

<a name="h.xja03ev51qni"></a>i5-30-9  [pScr-RBS-GFP]

<a name="h.popdmbz54bhh"></a>i5-30-5 [CP-Cra] (wrong, redid 8/16)

<a name="h.rufx1ux1fjhd"></a>i5-30-7 [CP-Scr]

<a name="h.9awna8x2h0rd"></a>

<a name="h.mw6tnc4qqkw0"></a>Miniprep

<a name="h.si7nyr3khs9e"></a>i5-30-1 → 53.2 [pFru-RBS-GFP]

<a name="h.xmkvqj7cif62"></a>i5-30-8 → 82.0  [pScr-RBS-GFP]

<a name="h.xja03ev51qni"></a>i5-30-9 → 92.3

<a name="h.popdmbz54bhh"></a>i5-30-5 → 78.2

<a name="h.mzxfdsv7dj3u"></a>i5-30-7 → 56.8

<a name="h.bb2xhfn87ro0"></a>Ligation

<a name="h.kw05m9lhb1km"></a>

<a href="#" name="52cc767bd298850806f2de9dd11c9f4d6597834f"></a><a href="#" name="40"></a><tbody></tbody>

<a name="h.cfli8oc53zij"></a>

<a name="h.tnl4yi53q6q5"></a>ComA

<a name="h.s672t8di70x2"></a>ComA

<a name="h.xamf4p3sdygb"></a>ComD

<a name="h.xhqfijsu5a81"></a>ComD

<a name="h.iodh2qkxwijg"></a>ComE

<a name="h.q0dxynqaozbt"></a>ComE

<a name="h.1jdaufvz7q36"></a>Dex

<a name="h.wqiqbk8r8gd1"></a>

<a name="h.7qpzbext29gi"></a>3:1

<a name="h.paqkalvi7ivq"></a>0:1

<a name="h.py5u8i4ld045"></a>3:1

<a name="h.98umyyb6hufi"></a>0:1

<a name="h.4zn9fywnaldl"></a>3:1

<a name="h.bowuoxuc9v03"></a>0:1

<a name="h.b0bqolnt4a82"></a>3:1

<a name="h.wqiqbk8r8gd1"></a>Insert

<a name="h.cxlvspvsbbq6"></a>10

<a name="h.cx070p8ko9dq"></a>0

<a name="h.mxqfbwczspx9"></a>6

<a name="h.x6sl1wi2rmrg"></a>0

<a name="h.j32dv9vyty22"></a>8

<a name="h.5y5ferwcv6x6"></a>0

<a name="h.vq89odwvolxf"></a>6.75

<a name="h.ncf56p5a20ln"></a>Vector

<a name="h.x79eciedmwz0"></a>3

<a name="h.ye8nbvfo344y"></a>3

<a name="h.oql65oi32idv"></a>5

<a name="h.l0e0tk65fc7h"></a>5

<a name="h.bqxjrvhy0zyn"></a>2.5

<a name="h.fzqgneabxu1r"></a>2.5

<a name="h.gbqzzss4ic"></a>2

<a name="h.w5xhsa8ln4s"></a>Buffer

<a name="h.o6exq9icnoew"></a>2

<a name="h.8lvpkmnzfss9"></a>2

<a name="h.n1eas5or9s5"></a>2

<a name="h.f1brl88flh57"></a>2

<a name="h.h6f75tjy0f0x"></a>2

<a name="h.cbk9vwmh6eq4"></a>2

<a name="h.5sp623sblow0"></a>2

<a name="h.side06m7hs1z"></a>Ligase

<a name="h.vf7x1ponl3ce"></a>1

<a name="h.57ieivf2yila"></a>1

<a name="h.nlpzer8ivilv"></a>1

<a name="h.9qwnd76rc8te"></a>1

<a name="h.yzv148wv3jtn"></a>1

<a name="h.xq801lqz9aqz"></a>1

<a name="h.t1kzlcyz6tp0"></a>1

<a name="h.eryxzcf41if6"></a>Water

<a name="h.oin1a99r6bel"></a>4

<a name="h.o27rrghxlr2r"></a>14

<a name="h.x8hkiphev4lz"></a>6

<a name="h.iemp08k16607"></a>12

<a name="h.ofephv51plgo"></a>6.5

<a name="h.1bamdauhn2ub"></a>14.5

<a name="h.uk544tp0w364"></a>8.25

<a name="h.w8c2gngxwsbd"></a>Total

<a name="h.4fo03sz9g275"></a>20

<a name="h.dp33idhscmtm"></a>20

<a name="h.sk7070l7diu1"></a>20

<a name="h.ecmr2drb5drx"></a>20

<a name="h.gbkwro9b8qff"></a>20

<a name="h.nyhm7ymibitx"></a>20

<a name="h.9t06fea2a72p"></a>20

<a name="h.557lenwh4acz"></a>

<a name="h.oqmynkqtai6z"></a>Insert: i5-25-3

<a name="h.bnwzdca05ak9"></a>Vector: i5-31-1

<a name="h.oqmynkqtai6z"></a>I: i5-25-3

<a name="h.bnwzdca05ak9"></a>V: i5-31-1

<a name="h.ya3rzcfa7g47"></a>I: i5-25-1

<a name="h.kca81p3gmj9k"></a>V:i5-31-2

<a name="h.ya3rzcfa7g47"></a>I: i5-25-1

<a name="h.kca81p3gmj9k"></a>V:i5-31-2

<a name="h.qauaamczt8gp"></a>I: i5-25-2

<a name="h.8li0bnudb2c4"></a>V: i5-31-3

<a name="h.qauaamczt8gp"></a>I: i5-25-2

<a name="h.8li0bnudb2c4"></a>V: i5-31-3

<a name="h.qauaamczt8gp"></a>I: i5-31-5

<a name="h.8li0bnudb2c4"></a>V: i5-31-2

<a name="h.edscmbgtd87j"></a>

<a name="h.224van5pf5l1"></a>1

<a name="h.382dg1rr800e"></a>2

<a name="h.tid93xvoiipv"></a>3

<a name="h.epzohjyq4x2c"></a>4

<a name="h.n2no183w2p1q"></a>5

<a name="h.q15mpoqb3srg"></a>6

<a name="h.oecqb87ui6x9"></a>7

<a name="h.p3lwslkhszap"></a>

<a name="h.o9ndf69qcz4j"></a>Transformation

<a name="h.w2q77lo5hvc1"></a>1 →ComA 3:1

<a name="h.720ozsgw6h93"></a>2→ComA 0:1

<a name="h.83lcczouup6g"></a>3→ComD3:1

<a name="h.onc7rknpledz"></a>4→ComD 0:1

<a name="h.4ficwey6o8ig"></a>5→ComE 3:1

<a name="h.6l83pdf75g42"></a>6→ComE 0:1

<a name="h.mq17t4ufsjk"></a>7→Dex → 1 colony

<a name="h.d6xm84vr4x83"></a>8→ + control → 100+ colonies

<a name="h.afwnx03l7tn1"></a>

<a name="h.7qzdi2w8gp73"></a>Test Digestion

<a name="h.9ijopada3shk"></a>

<a href="#" name="729b1547a7bce2086e0405f840e06e2d6f2617fc"></a><a href="#" name="41"></a><tbody></tbody>

<a name="h.ho1jt6iq2h8k"></a>

<a name="h.pkf1jsm450zm"></a>i5-30-1

<a name="h.7el0px6le1a7"></a>i5-30-5

<a name="h.24ib2ir6m2ox"></a>i5-30-7

<a name="h.u9z7nr8b8j0l"></a>i5-30-8

<a name="h.jd3f1732uftp"></a>i5-30-9

<a name="h.hjif9nafr6s5"></a>DNA

<a name="h.b8195jnfhmyt"></a>4

<a name="h.g2vpeq68qpjz"></a>3

<a name="h.tlcg0csj8q9o"></a>4

<a name="h.vtyhzhp29j1s"></a>3

<a name="h.n4fls87mzdah"></a>2

<a name="h.ta72xr1vgm6g"></a>Buffer

<a name="h.6pfp49qxzz2z"></a>2

<a name="h.an7yur76434y"></a>2

<a name="h.bmnsoogx6kls"></a>2

<a name="h.4m15zdyya5rb"></a>2

<a name="h.jg1wwznu9h7i"></a>2

<a name="h.hbxgu9ja18rw"></a>EcoRI

<a name="h.k4z0oqwlyqpk"></a>1

<a name="h.b8wnd3bwqon6"></a>1

<a name="h.548nat6b9w6j"></a>1

<a name="h.8i4o4jvawxr1"></a>1

<a name="h.ogg33qbohcgv"></a>1

<a name="h.5eqsi863qd7m"></a>PstI

<a name="h.9ie5iuta43y9"></a>1

<a name="h.ip7ekaxu5f2r"></a>1

<a name="h.1p411tww0gjc"></a>1

<a name="h.af9dcgo9r8lg"></a>1

<a name="h.8slr5xs1f1d"></a>1

<a name="h.7svg6bh9t6le"></a>Water

<a name="h.5mtz6i934nq2"></a>12

<a name="h.mdut81am6251"></a>13

<a name="h.qfgs3ysmz2q9"></a>12

<a name="h.6r9hcb7b6zdq"></a>13

<a name="h.2pj0e6fg6xdk"></a>14

<a name="h.h4hom3e1ujw3"></a>Total

<a name="h.4706ttpwek2z"></a>20

<a name="h.ml9hn9pp61rx"></a>20

<a name="h.k8veicqw4kv"></a>20

<a name="h.apq0moj322ez"></a>20

<a name="h.rxse6jl2tsge"></a>20

<a name="h.ygicqrth68qz"></a>

<a name="h.8hp55y1g01wx"></a>8/13

<a name="h.yx6z65elmr5b"></a>Repeat Digestion of CP medium from 8/8

<a name="h.bwm7rqkds14m"></a>

<a href="#" name="a476edab86376cacb30105a760f9e0bc715b499a"></a><a href="#" name="42"></a><tbody></tbody>

<a name="h.dv5qx0favqqa"></a>

<a name="h.nz82xc7e01g6"></a>i5-25-7

<a name="h.7vv70o4k78pk"></a>DNA

<a name="h.lgu14qf36qfo"></a>20

<a name="h.u1swwb9q8awq"></a>Buffer

<a name="h.5i7qfzvnj3pl"></a>5

<a name="h.qrzhx6mr6wwb"></a>PstI

<a name="h.1088ioza8a33"></a>1

<a name="h.9p92448b6d1q"></a>SpcI

<a name="h.gv2zsmjthhee"></a>1

<a name="h.d132mkn7dsri"></a>Water

<a name="h.qewdkta6kgva"></a>13

<a name="h.huyfpddhtgtt"></a>Total

<a name="h.sfc9bdbspbdo"></a>50

<a name="h.q07fj8aatkj8"></a>                        → i5-32-1 13.1 ng/ul

<a name="h.83a11ejiq1bl"></a>

<a name="h.h0fq67pbrtou"></a>Ligation

<a name="h.zb8ws8jzfrz3"></a>

<a href="#" name="f7921980145d7b0a2a6dc1f0e92ecc8cfbba7c5a"></a><a href="#" name="43"></a><tbody></tbody>

<a name="h.x1caggew13f2"></a>

<a name="h.sdc6rgdv6jlk"></a>Insert: i5-26-8 [Cra]

<a name="h.ow488913hovq"></a>Vector: i5-32-1 [CP]

<a name="h.9hn0spu7m8ih"></a>

<a name="h.s5a78zhhvqcc"></a>3:1

<a name="h.760n4t6ht1gc"></a>0:1

<a name="h.9hn0spu7m8ih"></a>insert

<a name="h.xw2xpb6fx4ou"></a>4.36

<a name="h.olxy8kdv8cnu"></a>0

<a name="h.93kp6ku0iiwh"></a>Vector

<a name="h.rcvg4ygk32zo"></a>3.8

<a name="h.5wmx0igowyu6"></a>3.8

<a name="h.1wsohk9s5r10"></a>Buffer

<a name="h.rfm565dvbc4t"></a>2

<a name="h.rvag37m6puj4"></a>2

<a name="h.pmalz0lmrokt"></a>ligase

<a name="h.pwctjlc04whd"></a>1

<a name="h.f9a1d51d1dmr"></a>1

<a name="h.qa6yiisdvkdk"></a>Water

<a name="h.qk6vu2naq0r6"></a>8.8

<a name="h.yqm7u1tkmdtd"></a>13.2

<a name="h.a3clo8qmlbds"></a>total

<a name="h.z59eeer6r1se"></a>20

<a name="h.5rvon3dlqqxt"></a>20

<a name="h.vn9sjwmsj6xx"></a>

<a name="h.jsrsefbvapq8"></a>1

<a name="h.ag5akp7a4jps"></a>2

<a name="h.1tvhctn67d92"></a>

<a name="h.qzwgdl1ntt7d"></a>

<a href="#" name="2a6e9d48bb41b4c2480d25a13c187b1c87c3892a"></a><a href="#" name="44"></a><tbody></tbody>

<a name="h.ipdc1yd24vxf"></a>

<a name="h.f4wjlhm56vu7"></a>Insert: i5-26-9 [Scr]

<a name="h.f4wjlhm56vu7"></a>Vector: i5-31-1 [CP]

<a name="h.qh1y06b3479c"></a>

<a name="h.48wssfx7r1u7"></a>3:1

<a name="h.48wssfx7r1u7"></a>0:1

<a name="h.9hn0spu7m8ih"></a>insert

<a name="h.b11zavitsha"></a>5.13

<a name="h.b11zavitsha"></a>0

<a name="h.93kp6ku0iiwh"></a>Vector

<a name="h.uq46wo93oxow"></a>3.8

<a name="h.uq46wo93oxow"></a>3.8

<a name="h.1wsohk9s5r10"></a>Buffer

<a name="h.ivm0seypj81u"></a>2

<a name="h.ivm0seypj81u"></a>2

<a name="h.pmalz0lmrokt"></a>ligase

<a name="h.b4bsa7b2vnai"></a>1

<a name="h.b4bsa7b2vnai"></a>1

<a name="h.qa6yiisdvkdk"></a>Water

<a name="h.28sx6m74nth0"></a>8

<a name="h.28sx6m74nth0"></a>13.2

<a name="h.a3clo8qmlbds"></a>total

<a name="h.pqek5guz4619"></a>20

<a name="h.pqek5guz4619"></a>20

<a name="h.ebecajg2zi5f"></a>

<a name="h.bzw6gpt1ozvt"></a>3

<a name="h.bzw6gpt1ozvt"></a>4

<a name="h.vf0fvkdmnzqx"></a>

<a name="h.q9a8up4ldyhk"></a>Transformation

<a name="h.arfeyr3nrwf1"></a>1 → i5-33-1 → 0 colonies

<a name="h.7j7e2d5zvfnv"></a>2 → i5-33-2 → 0 colonies

<a name="h.tj6n93n3hd83"></a>3 → i5-33-3 → 0 colonies

<a name="h.lj3xj5v1afoj"></a>4→ i5-33-4 → 0 colonies

<a name="h.mqacxtcn19jp"></a>Positive Control (i5-22-2) → 0 colonies

<a name="h.2d1ug8400cez"></a>Retransformation of strong CP fro 8/16 → i5-33-5 → 50 + colonies picked 2 colonies → i5-33-6

<a name="h.m5cstemmlke7"></a>                                                                                        i5-33-7

<a name="h.dk6cgw8yaj3l"></a>

<a name="h.qw529owinve4"></a>8/14

<a name="h.6s52vkatmbqc"></a>Made Glycerol stock of:

<a name="h.733nppqgji5e"></a>Dex (i5-33-8)

<a name="h.tupe8rjtea8j"></a>

<a name="h.2n5f35kjdexk"></a>Miniprep/ Glycerol stock

<a name="h.56b95g7u2c82"></a>i5-33-8 Dex → 154

<a name="h.l505z58uct98"></a>i5-33-9 Com A-1 → 81.8

<a name="h.woxm5mh4s8yx"></a>i5-33-10 Com D-1 → 70.5

<a name="h.7dy67k57t83u"></a>i5-33-11 Com E-1 → 97.7

<a name="h.j67czes1nje3"></a>i5-33-12 Com A-2 → 77.6

<a name="h.8o82cq131mwq"></a>i5-33-13 Com D-2 → 47.3

<a name="h.nhsd22vcnxzk"></a>i5-33-14 Com E-2 → 127. 5

<a name="h.6qqlcwmj894y"></a>

<a name="h.1vzgbdfgusl"></a>Transformation redo from 8/13

<a name="h.d9xabqe163og"></a>1 → i5-34-1

<a name="h.ajqqufir100x"></a>2 → i5-34-2

<a name="h.mala9ohzl2"></a>3→ i5-34-3         [All colonies counts next page]

<a name="h.5u9mnvrjowzf"></a>4→ i5-34-4

<a name="h.7lp05xi8o5jz"></a>i5-10-1 → positive control

<a name="h.20jfauarvdlp"></a>

<a name="h.owy03xy20j5h"></a>Test Digestion

<a name="h.f6q2bhpdazf5"></a>

<a href="#" name="763ae82b5bad88d9b75907a8b6179cfa0b4e3ecb"></a><a href="#" name="45"></a><tbody></tbody>

<a name="h.lxz4mxrdunih"></a>

<a name="h.v9xpuald20wk"></a>i5-33-8

<a name="h.ci3rk05ry5ry"></a>DNA

<a name="h.x9gcwu5g13mr"></a>2

<a name="h.36xpyj93xpyr"></a>Cutsmart

<a name="h.70xva2p118jk"></a>2

<a name="h.n2y7cvs0db2t"></a>EcoRI

<a name="h.j3mb0eg4shbn"></a>1

<a name="h.qq9rejw4pt8y"></a>PstI

<a name="h.f2q0gtusxx5h"></a>1

<a name="h.ru7ua7fjbnn"></a>Water

<a name="h.7gvq28yviv4r"></a>14

<a name="h.c2bhr457dozj"></a>Total

<a name="h.dzzcigrpxzz9"></a>20

<a name="h.9bt0nbe8x9jf"></a>

<a name="h.vl55fgfq33t8"></a>Test Digestion

<a name="h.us9lwo4derb9"></a>

<a href="#" name="8e88e4342ef8671748c05701d136be4b2862588e"></a><a href="#" name="46"></a><tbody></tbody>

<a name="h.dzj8kdmrw064"></a>

<a name="h.fx2prjlkq76j"></a>i5-33-9

<a name="h.7ht2krvpioy7"></a>i5-33-10

<a name="h.my0t2hlhzat1"></a>i5-33-11

<a name="h.93llo9118vsg"></a>i5-33-12

<a name="h.w0foxt8aex4d"></a>i5-33-13

<a name="h.fd58o8cixmp9"></a>i5-33-14

<a name="h.8t01v6p6g6oc"></a>DNA

<a name="h.n60btcbhfv6l"></a>3

<a name="h.56u2amgcing2"></a>3

<a name="h.uweeq9qxi7z5"></a>3

<a name="h.dhva6bj8wovx"></a>3

<a name="h.i0w5nlihhkey"></a>3

<a name="h.akr7qbkgb7rs"></a>3

<a name="h.1o0slz60o70b"></a>Cutsmart

<a name="h.diuyuwnqt0t0"></a>2

<a name="h.jzdj4uaceps"></a>2

<a name="h.591d5yr5feug"></a>2

<a name="h.yym2agfwpqlm"></a>2

<a name="h.l4b28dk2yyp4"></a>2

<a name="h.hfxdq2uysvok"></a>2

<a name="h.kabmxhl1q7p5"></a>EcoRI

<a name="h.g5a1h31nsq8z"></a>1

<a name="h.t0amuaxmmile"></a>1

<a name="h.fk9ozzb1qlgo"></a>1

<a name="h.fvctb7h3kyk6"></a>1

<a name="h.2b5qhqv78tp1"></a>1

<a name="h.crm4mgms332k"></a>1

<a name="h.kabmxhl1q7p5"></a>PstI

<a name="h.g5a1h31nsq8z"></a>1

<a name="h.t0amuaxmmile"></a>1

<a name="h.fk9ozzb1qlgo"></a>1

<a name="h.fvctb7h3kyk6"></a>1

<a name="h.2b5qhqv78tp1"></a>1

<a name="h.crm4mgms332k"></a>1

<a name="h.5hmye17dbm75"></a>Water

<a name="h.yc85jwx5iojg"></a>13

<a name="h.bdtwpekf68tl"></a>13

<a name="h.z1er9hn2id7m"></a>13

<a name="h.405i5cnqv9jx"></a>13

<a name="h.q31bwpoozg1v"></a>13

<a name="h.bwp6ypriegfa"></a>13

<a name="h.z79365d72210"></a>Total

<a name="h.x1isskclwh35"></a>20

<a name="h.p8rtm5wyi0xu"></a>20

<a name="h.hu038w9qqu68"></a>20

<a name="h.7tr7u75nb19w"></a>20

<a name="h.4reavinajn18"></a>20

<a name="h.f1ewrk9uxa8h"></a>20

<a name="h.goheppc2dx61"></a>Results: inconclusive

<a name="h.ml1ph92etfaa"></a>

<a name="h.si5hs73lcmbi"></a>Repeat Test Digestion

<a name="h.q0m4prd27fxl"></a>

<a href="#" name="cf0002eb5efaacd136af2d718bc509f859fa78d2"></a><a href="#" name="47"></a><tbody></tbody>

<a name="h.ldjmfp5uvq51"></a>

<a name="h.jj7751sgt03e"></a>i5-33-8

<a name="h.r5ck60k1i6yt"></a>DNA

<a name="h.nit2z0jd4st"></a>2

<a name="h.b079rgaxf3ng"></a>Cutsmart

<a name="h.ufdry88xjj1e"></a>2

<a name="h.gnrr87dmenoa"></a>SpcI

<a name="h.8x3hmh3on29p"></a>1

<a name="h.c8qhrq7nxqpl"></a>XbaI

<a name="h.d7r04bsp65ij"></a>1

<a name="h.754zetd4eibj"></a>Water

<a name="h.ea765ldv4x3v"></a>14

<a name="h.so7akcxwhrno"></a>Total

<a name="h.l0rch97n5392"></a>20

<a name="h.a89iq8grwvpc"></a>Failed

<a name="h.aft4hmllxw6"></a>

<a name="h.1fl9kmdwiq0s"></a>8/15

<a name="h.9pnxeap1km09"></a>i5-34-1 →  100+ colonies → picked 2 colonies → i5-35-5 [CP(M)-Cra]

<a name="h.h8ns16axtbxy"></a>                                                i5-35-6

<a name="h.rnj1u24eoh1n"></a>i5-34-2 → 0 colonies

<a name="h.hwzvnqy1v49y"></a>i5-34-3 → 50 + colonies → picked 2 colonies → i5-35-3 [CP(M)-Scr]

<a name="h.v0bhzs2t7dg1"></a>                                                i5-35-4

<a name="h.o6k2epid3y1m"></a>i5-34-4 → 2 colonies

<a name="h.lqkxekytz533"></a>+ control → 0 colonies

<a name="h.ddn15or0pvjt"></a>

<a name="h.eb99bx8tsmo"></a>Miniprep And Glycerol Stock made

<a name="h.wgkh4hqdjib9"></a>i5-33-6 → 53.1 ng/ul        [CP strong]

<a name="h.2l9z0z37ujxp"></a>i5-33-7 → 58.9 ng/ul        [CP strong]

<a name="h.jbiobo9ztdl3"></a>

<a name="h.15mzsqz3n0ly"></a>Digestion

<a name="h.wiyqpncv0j5"></a>

<a href="#" name="05dc6e69edb1189742c67d82839f72d087945dae"></a><a href="#" name="48"></a><tbody></tbody>

<a name="h.bdgsnrytul6"></a>

<a name="h.w9z5jrg1x57h"></a>i5-33-6 (CP strong)

<a name="h.b0c0fmtlkt3f"></a>DNA

<a name="h.91w4r2cdnt0t"></a>30

<a name="h.yig94fpjns1e"></a>NEBuffer

<a name="h.sr5eiumlgmbu"></a>5

<a name="h.9ltstaxfarc7"></a>PstI

<a name="h.s73qz9hfistn"></a>1

<a name="h.u5ilp2lcp5bj"></a>SpeI

<a name="h.wuokcmecm27d"></a>1

<a name="h.ngg479drtbhh"></a>Water

<a name="h.67e3o1g0j5jj"></a>13

<a name="h.gbd3s8pck0vp"></a>Total

<a name="h.3bk31q29c9gh"></a>50

<a name="h.d7zb8v4im8lz"></a>        i5-35-1 → 13.9 ng/ul

<a name="h.8v3us1gmtd5t"></a>

<a href="#" name="c7601e78722b9028483841b806f3352b315864f3"></a><a href="#" name="49"></a><tbody></tbody>

<a name="h.6fxhopel4kth"></a>

<a name="h.thvwwy6o83wu"></a>i5-10-1 (RBS-GFP)

<a name="h.lri2snfhcour"></a>DNA

<a name="h.hdc1walngwa5"></a>20

<a name="h.js8kzetr99dk"></a>NEBuffer

<a name="h.yw9l8h6wfus8"></a>5

<a name="h.wxr4meh0e6gj"></a>PstI

<a name="h.l6mmbvsgibh8"></a>1

<a name="h.ounargsrp2qk"></a>XbaI

<a name="h.hez37ql5nv4b"></a>1

<a name="h.grnbmt70wwmu"></a>Water

<a name="h.s8i2ruautrt4"></a>23

<a name="h.mhmxk7kbzx91"></a>Total

<a name="h.e8vmy2mwh768"></a>50

<a name="h.e9bxlwesm3h9"></a>        i5-35-2 → 17.1 ng/ul

<a name="h.91z0p3x2ca36"></a>

<a name="h.37hiui2t0eqr"></a>PCR

<a name="h.v3tapuz1tbpk"></a>

<a href="#" name="9a5b80d5d6e672c917e435dbf711ed639d188cef"></a><a href="#" name="50"></a><tbody></tbody>

<a name="h.luigpv11kti0"></a>Primers

<a name="h.i83ocmyj89ou"></a>4

<a name="h.onsriba2lcts"></a>10

<a name="h.8ck7zl3t01qq"></a>DNA

<a name="h.vafajfrzu42a"></a>1

<a name="h.d3tpned157iv"></a>1

<a name="h.lojiwgflre5e"></a>F

<a name="h.n76arnyhqnaz"></a>1

<a name="h.nv47yawjriya"></a>1

<a name="h.ohyt97edpj3r"></a>R

<a name="h.l47l0eaq5ibc"></a>1

<a name="h.ufvoe9hrrvr2"></a>1

<a name="h.7q0yr9mz7mxs"></a>Phusion

<a name="h.c0zjhnj8bt96"></a>10

<a name="h.11rmx5egotds"></a>10

<a name="h.vfwxkawdm79z"></a>Water

<a name="h.14kzu2xq6ksa"></a>7

<a name="h.vo12j22ezux6"></a>7

<a name="h.tp7f6moiu9o7"></a>Tota

<a name="h.vigfadqoy52m"></a>20

<a name="h.pj1g9enicib4"></a>20

<a name="h.icv4fyizyb8t"></a>

<a name="h.e6ztamlui3vf"></a>PCR Purification

<a name="h.tzxi0owohar5"></a>1→ 24.4 ng/ul        ComA

<a name="h.egr67g98m24o"></a>2→ 43.6 ng/ul         Dex

<a name="h.5raa3sfs3ku3"></a>

<a name="h.nh4olqlh59mv"></a>Digestion

<a name="h.mhfjozi9fu80"></a>

<a href="#" name="9d3ffed307af2dbd498918039490954896a3ee76"></a><a href="#" name="51"></a><tbody></tbody>

<a name="h.efof4gy1vxzj"></a>

<a name="h.1z35g8cwhrxr"></a>ComA

<a name="h.2f6rme6cumya"></a>Dex

<a name="h.6w8x1plhemba"></a>DNA

<a name="h.8wptridw7fm"></a>30

<a name="h.v1ye82gh2een"></a>30

<a name="h.quisp77awysu"></a>Buffer

<a name="h.f69r8p31sowc"></a>5

<a name="h.cyk3clpfnky7"></a>5

<a name="h.x0qk4inxyt7n"></a>E

<a name="h.p2ywfsdycopq"></a>1

<a name="h.ndmxu8jdazmm"></a>0

<a name="h.x4glb5qnh54p"></a>X

<a name="h.rpa1pcys26m4"></a>0

<a name="h.8vd9ag7mii8q"></a>1

<a name="h.v19rlpfqg53s"></a>S

<a name="h.2cxf3tm6bc"></a>1

<a name="h.me88l1uu0xpd"></a>1

<a name="h.x7o6sjj1sxsq"></a>Water

<a name="h.fuj809w7vpkf"></a>13

<a name="h.laffqtto3mjy"></a>13

<a name="h.rm77uats1phh"></a>Total

<a name="h.z9ejdad5bi27"></a>50

<a name="h.5qrz284vjn73"></a>50

<a name="h.x1e079w76rvn"></a>        i5-36-1                        i5-36-2

<a name="h.2g7aby6b4sja"></a>

<a name="h.7ikwtgti8hd5"></a>Ligation

<a name="h.2ortfrl8j2sp"></a>

<a href="#" name="1227c9c6f403d6c0a3cacde33274b481d477f1e4"></a><a href="#" name="52"></a><tbody></tbody>

<a name="h.446u184cvrbf"></a>

<a name="h.ja75d7spkaqi"></a>Insert: i5-35-2

<a name="h.k6zu2ge2aals"></a>Vector: i5-26-7

<a name="h.ais0p8pbx53e"></a>

<a name="h.7u0t2140hcem"></a>3:1

<a name="h.61xlumz88osz"></a>0:1

<a name="h.ais0p8pbx53e"></a>Insert

<a name="h.o2vcyjd38d91"></a>3.4

<a name="h.2fthvm98b3is"></a>0

<a name="h.y7nq0i4hddjb"></a>Vector

<a name="h.gr4k6liyrm8f"></a>2

<a name="h.3od12bj6jqka"></a>2

<a name="h.7f9fqw9tfe83"></a>Buffer

<a name="h.78rj1ra7fpk7"></a>2

<a name="h.rzwwuegmcbg3"></a>2

<a name="h.7bcr7zyep0ao"></a>Ligase

<a name="h.26hp2wrkq5us"></a>1

<a name="h.8mjqylqwwqms"></a>1

<a name="h.ou681b2fez89"></a>Water

<a name="h.3m8taamd7nxu"></a>11.6

<a name="h.qpstgtinnx2y"></a>15

<a name="h.gxspftcsh4ih"></a>Total

<a name="h.q9oa3rbhng9g"></a>20

<a name="h.kbwez6myde3b"></a>20

<a name="h.tbjc9f9wah6k"></a>

<a name="h.wawvtmqqmgs4"></a>1

<a name="h.eu425i3oyc1y"></a>2

<a name="h.rluy16t8b52j"></a>        

<a href="#" name="fee9accc571522d9f797ac889a962b7e92f1c9f9"></a><a href="#" name="53"></a><tbody></tbody>

<a name="h.7r205nsg42zm"></a>

<a name="h.3vi5fw695hr3"></a>Insert: i5-35-2

<a name="h.hwa5u13k1iwv"></a>Vector: i5-32-1

<a name="h.1bvzbhv2uwc9"></a>

<a name="h.am7cktusj87g"></a>3:1

<a name="h.18tkuz7w6jlv"></a>0:1

<a name="h.ais0p8pbx53e"></a>Insert

<a name="h.r26r5wp2h3zy"></a>3.4

<a name="h.av3s0e2o73ag"></a>0

<a name="h.y7nq0i4hddjb"></a>Vector

<a name="h.485wyvn50xr1"></a>3.8

<a name="h.rmtoc1k3iwgv"></a>3.8

<a name="h.7f9fqw9tfe83"></a>Buffer

<a name="h.f2odavphjx44"></a>2

<a name="h.d8fc3j8vu66d"></a>2

<a name="h.7bcr7zyep0ao"></a>Ligase

<a name="h.1jp365pqla93"></a>1

<a name="h.2chpmzvl7gyu"></a>1

<a name="h.ou681b2fez89"></a>Water

<a name="h.e2k95lv8a1o6"></a>9.8

<a name="h.e7b88jjs85vg"></a>13.2

<a name="h.gxspftcsh4ih"></a>Total

<a name="h.wh7bbpdbgw1u"></a>20

<a name="h.xaqtpwlmq1op"></a>20

<a name="h.agdulkfcjm9t"></a>

<a name="h.4n6g30d55mp"></a>3

<a name="h.6zst6h5r0ejn"></a>4

<a name="h.mb93eitj4hsc"></a>

<a href="#" name="386ac964dd8806146fbe6faa982da9292dabbd15"></a><a href="#" name="54"></a><tbody></tbody>

<a name="h.nrlnl2uh09mn"></a>

<a name="h.x49urbrz3psl"></a>Insert: i5-35-2

<a name="h.igj46wb0fylf"></a>Vector: i5-35-1

<a name="h.pi287kbft10o"></a>

<a name="h.1tqf0d29nwo3"></a>3:1

<a name="h.6odrzlxut793"></a>0:1

<a name="h.ais0p8pbx53e"></a>Insert

<a name="h.dvpf17uz7zb4"></a>3.4

<a name="h.1pwj8dtfqvjf"></a>0

<a name="h.y7nq0i4hddjb"></a>Vector

<a name="h.yzrt9o4no5x4"></a>3.8

<a name="h.6j7asswk37rx"></a>3.8

<a name="h.7f9fqw9tfe83"></a>Buffer

<a name="h.yobqbto1ibdx"></a>2

<a name="h.tcji8slkvpx0"></a>2

<a name="h.7bcr7zyep0ao"></a>Ligase

<a name="h.yd2rnm4y8y12"></a>1

<a name="h.pk7pom6d1u2u"></a>1

<a name="h.ou681b2fez89"></a>Water

<a name="h.t0f9rlc21ws"></a>9.8

<a name="h.k9wsifj3hu8y"></a>13.2

<a name="h.gxspftcsh4ih"></a>Total

<a name="h.7pomzfuh0526"></a>20

<a name="h.l7pqn4xi6uh1"></a>20

<a name="h.enkw485jp5hr"></a>

<a name="h.khiijy6wy0l"></a>5

<a name="h.a1d7jjrad43w"></a>6

<a name="h.f4jk5w90pd9d"></a>

<a name="h.2blu38ywme91"></a>i5-36-2 → 7.2

<a name="h.efpckwm48ckh"></a>i5-36-1 → 5.5

<a name="h.qclf1lg5nbj3"></a>

<a name="h.vsm37xfci8as"></a>Ligation

<a name="h.9zvycs9ruwav"></a>

<a href="#" name="b699df3897563c86ceb49c73d01dfb244fb43787"></a><a href="#" name="55"></a><tbody></tbody>

<a name="h.dxt9q8ssmb2i"></a>

<a name="h.frqh55sr2nnq"></a>insert: i5-36-1

<a name="h.ad8d23ff7t2"></a>Vector: i5-35-1

<a name="h.7k1j0y4fpakg"></a>

<a name="h.vef3x2rqx7dv"></a>3:1

<a name="h.dctwbc6t0l85"></a>0:1

<a name="h.ais0p8pbx53e"></a>Insert

<a name="h.ecvngv16j278"></a>14

<a name="h.um5s8ol7avca"></a>0

<a name="h.y7nq0i4hddjb"></a>Vector

<a name="h.8uecg72bbyyh"></a>2

<a name="h.hvlswlaxagkm"></a>2

<a name="h.7f9fqw9tfe83"></a>Buffer

<a name="h.tarmcxbd8xv"></a>2

<a name="h.g5t1s4dgbdel"></a>2

<a name="h.7bcr7zyep0ao"></a>Ligase

<a name="h.ri21cogkisnp"></a>1

<a name="h.8vvltxqxtq3f"></a>1

<a name="h.ou681b2fez89"></a>Water

<a name="h.85juk2himz11"></a>1

<a name="h.qizhmhurhfil"></a>15

<a name="h.gxspftcsh4ih"></a>Total

<a name="h.588l61v2wpyy"></a>20

<a name="h.ungk98tufb2i"></a>20

<a name="h.gqlikebq8qye"></a>

<a name="h.wv8bg4a2wc3u"></a>1

<a name="h.heh6q5m96vh0"></a>2

<a name="h.z2v87m60062"></a>

<a href="#" name="7a81143f15cd37b423cfe73c5985a4323703642c"></a><a href="#" name="56"></a><tbody></tbody>

<a name="h.537owfrpel2k"></a>

<a name="h.pnjlac6u93rt"></a>Insert: i5-36-2

<a name="h.jlnxtvctx31c"></a>Vector: i5-35-2

<a name="h.c2xoe0c2eh1n"></a>

<a name="h.l9j36qbonyaz"></a>3:1

<a name="h.t5y7pj1zrs9b"></a>0:1

<a name="h.ais0p8pbx53e"></a>Insert

<a name="h.8tisc44fdrm0"></a>11

<a name="h.2t2o6zak6asp"></a>0

<a name="h.y7nq0i4hddjb"></a>Vector

<a name="h.9hbux94l4his"></a>2

<a name="h.u94on5msviiy"></a>2

<a name="h.7f9fqw9tfe83"></a>Buffer

<a name="h.86ufhok2o4bg"></a>2

<a name="h.uxhxzp95ufr9"></a>2

<a name="h.7bcr7zyep0ao"></a>Ligase

<a name="h.rw15wrbffi7n"></a>1

<a name="h.vj2h9qly4owu"></a>1

<a name="h.ou681b2fez89"></a>Water

<a name="h.sqiowcllzkon"></a>4

<a name="h.3kah5vuq02rg"></a>15

<a name="h.gxspftcsh4ih"></a>Total

<a name="h.5gjl5p87tzyb"></a>20

<a name="h.g8zq8upbpurr"></a>20

<a name="h.grj8cm9qh59r"></a>

<a name="h.fj6uecrb8n39"></a>3

<a name="h.j8lc1i1cnvyf"></a>4

<a name="h.xoy1ifbetzcb"></a>

<a name="h.wknss8al3bw9"></a>Transformation

<a name="h.i3rp59nff5kj"></a>1 → i5-37-1 (3:1 CP weak-GFP) → 50+ colonies → picked 1 colony → i5-37-1

<a name="h.afr5apqmgh7x"></a>2→ i5-37-2 (0:1) → 0 colonies

<a name="h.6u95ndm7eryf"></a>3→ i5-37-3 (3:1 CP medium-GFP) → 20+ colonies → picked 1 colony → i5-37-3

<a name="h.8tjez9d9wwrq"></a>4→ i5-37-4 (0:1) → 0 colonies

<a name="h.gslcbbx4vbd0"></a>5→ i5-37-5 (3:1 CP strong- GFP) → 10 colonies → picked 1 colony → i5-37-5

<a name="h.nce9o8wtiv9r"></a>6→ i5-37-6 (0:1) → 0 colonies

<a name="h.thw8mfciqpze"></a>+ control with GFP (pLac-GFP) i5-11-5 → i5-37-7 → 200+ colonies → picked 1 colony → i5-37-7

<a name="h.5fzgywok5zsu"></a>+ control without GFP (pLac) i5-10-3 → i5-37-8 → 300+ colonies → picked 1 colony → i5-37-8

<a name="h.ho9zttwlrkrd"></a>

<a name="h.p7g4wz3741my"></a>8/16

<a name="h.xenm88qj8tr0"></a>Miniprep and Glycerol Stock made

<a name="h.w7e9rh2vqpkl"></a>i5-35-5 → 102.4 [CP medium -Cra]

<a name="h.zcm807cia938"></a>i5-35-6 → 96.8 [CP medium -Cra]

<a name="h.bx69nd5oum52"></a>i5-35-3 → 174.1 [CP medium -Sur]

<a name="h.hnfr01465w8h"></a>i5-35-4 → 81.9[CP medium -Sur]

<a name="h.cjlm4a10dfu3"></a>

<a name="h.5x45icvqjupb"></a>Ligation

<a name="h.j6vssugbvt7n"></a>

<a href="#" name="5a5e919e87b21cebb29d96bd7724740f71fac1d2"></a><a href="#" name="57"></a><tbody></tbody>

<a name="h.480jjn4ddaml"></a>

<a name="h.kgmh70g2y7xo"></a>Insert: i5-36-1

<a name="h.gsx3jhynwuks"></a>Vector: i5-31-1

<a name="h.9nx9wtt0euyr"></a>

<a name="h.vn8opwiae48j"></a>3:1

<a name="h.fphaff3udskg"></a>0:1

<a name="h.tvcfje28ne7o"></a>Vector

<a name="h.jjcthnhd65gc"></a>Insert

<a name="h.ue0m7uo64kig"></a>2

<a name="h.6wklvoawyj75"></a>14

<a name="h.4umn0ia7shi"></a>2

<a name="h.lrwxvelm8yr5"></a>0

<a name="h.eks6fegflllt"></a>Buffer

<a name="h.c052kfza5hvj"></a>2

<a name="h.lez442oz67q4"></a>2

<a name="h.igjte5dsmxv"></a>Ligase

<a name="h.84rlo2sfzavb"></a>1

<a name="h.g66311p9ogb8"></a>1

<a name="h.opzit7ttzhgr"></a>Water

<a name="h.sbiii5hnpqbv"></a>1

<a name="h.ykdcw8msca4d"></a>15

<a name="h.ln3bvpo676an"></a>Total

<a name="h.ivtvob47w6ky"></a>20

<a name="h.ebihepncp1fn"></a>20

<a name="h.l8iinr8zi0m2"></a>

<a name="h.uf0qm8vb8pk9"></a>1

<a name="h.b2amah8qzdry"></a>2

<a name="h.bbkojexwxf70"></a>

<a name="h.vghxd7c06693"></a>

<a href="#" name="e89e9ab2a79d4b7c0dfc327da0d0533b126297ad"></a><a href="#" name="58"></a><tbody></tbody>

<a name="h.x1d44wutcfux"></a>

<a name="h.p490enb55ood"></a>Vector: i5-31-2

<a name="h.f9s6zwrz984e"></a>Insert: i5-36-2

<a name="h.3ydbg5rxcnfw"></a>

<a name="h.iesw723u5fvk"></a>3:1

<a name="h.v57bmrvyii4f"></a>0:1

<a name="h.tvcfje28ne7o"></a>Vector

<a name="h.otvnii1tpwj6"></a>2

<a name="h.70mhhah07aza"></a>2

<a name="h.eks6fegflllt"></a>Insert

<a name="h.mxdk1e2t3mph"></a>11

<a name="h.yrn8y8ux1ost"></a>0

<a name="h.eks6fegflllt"></a>Buffer

<a name="h.nazn83xlqr16"></a>2

<a name="h.1n1692j4zc5f"></a>2

<a name="h.igjte5dsmxv"></a>Ligase

<a name="h.gk7uqqggkn8j"></a>1

<a name="h.vw4m32eo9wed"></a>1

<a name="h.opzit7ttzhgr"></a>Water

<a name="h.qsebwi85qwlx"></a>4

<a name="h.uuu3n62mo9sq"></a>15

<a name="h.ln3bvpo676an"></a>Total

<a name="h.2z45cb58uzve"></a>20

<a name="h.1u63u2j7cbs5"></a>20

<a name="h.weyf48fo89s5"></a>

<a name="h.b393zp9achik"></a>3

<a name="h.x2s8ejdapab3"></a>4

<a name="h.79vsjsq0ozaz"></a>

<a name="h.bck05rrifjsn"></a>Digestion

<a href="#" name="da87f34366964cbda2c79064dd7a5557c2a56ccf"></a><a href="#" name="59"></a><tbody></tbody>

<a name="h.cp3drxo02zlu"></a>

<a name="h.9bgajhef44mo"></a>i5-35-5

<a name="h.ilytc521q7z8"></a>i5-35-3

<a name="h.ftgy4652e3jj"></a>DNA

<a name="h.4zwquazf9r00"></a>20

<a name="h.5vkb6663ecbc"></a>20

<a name="h.cw8ungggm5i2"></a>NEBuffer

<a name="h.n97w1d3ne244"></a>5

<a name="h.6r3hr3ocjrlh"></a>5

<a name="h.5cwj9i1t3fn8"></a>PstI

<a name="h.cn1rhr6fozee"></a>1

<a name="h.idpxjh190o22"></a>1

<a name="h.drgnwxr8oakf"></a>SpeI

<a name="h.8tw8lhpig6pm"></a>1

<a name="h.p7gjrzcrha61"></a>1

<a name="h.t579oqr0nmx4"></a>Water

<a name="h.gelxflpj4r9o"></a>13

<a name="h.rhr78tydnl0v"></a>13

<a name="h.16nz6uq6ddrr"></a>Total

<a name="h.622yhn26s54o"></a>50

<a name="h.ywwgyfb4gcot"></a>50

<a name="h.5pgdl8fqw7i"></a>

<a name="h.5httc9x9d6rz"></a>i5-38-5

<a name="h.ybu75mfzznxf"></a>i5-38-6

<a name="h.k5jbwb3fp85x"></a>

<a name="h.xwh3vt5mhwz6"></a>

<a href="#" name="d16ddbe4f852fdb8c9032948186c12b1c32aae69"></a><a href="#" name="60"></a><tbody></tbody>

<a name="h.yexdchbtvwmh"></a>

<a name="h.a0ub1rdwnu0m"></a>i5-30-1

<a name="h.oorpyfrunmgr"></a>i5-30-8

<a name="h.ftgy4652e3jj"></a>DNA

<a name="h.xdo1mb5698j4"></a>20

<a name="h.rwx9ye92aef"></a>20

<a name="h.cw8ungggm5i2"></a>NEBuffer

<a name="h.8uwpbjiox103"></a>5

<a name="h.y6ti414jt9dj"></a>5

<a name="h.5cwj9i1t3fn8"></a>PstI

<a name="h.dtp9b4osul7y"></a>1

<a name="h.b0sf5zoszj33"></a>1

<a name="h.drgnwxr8oakf"></a>XbaI

<a name="h.1b0mlm3ivl2e"></a>1

<a name="h.tjl2sgugzhus"></a>1

<a name="h.t579oqr0nmx4"></a>Water

<a name="h.lsjz7a77eu04"></a>13

<a name="h.qba6p47y043k"></a>13

<a name="h.16nz6uq6ddrr"></a>Total

<a name="h.vpq2fyh1dpf4"></a>50

<a name="h.y1b7c1a8jlet"></a>50

<a name="h.m8tt9g2ks5iq"></a>

<a name="h.nog0hulwra5m"></a>i5-38-7

<a name="h.bqlxrvbujnv4"></a>i5-38-8

<a name="h.8ky659ftgrfe"></a>

<a name="h.1cho3s998m0m"></a>Transformation

<a name="h.42b7i439vc6k"></a>i5-36-1 3:1 → i5-38-1 → 30 colonies → picked 2 colonies →  i5-38-9

<a name="h.a6yy48ute5r8"></a>                                                        i5-38-10

<a name="h.xsfqvvxh8333"></a>i5-36-1 0:1 → i5-38-2 → 8 colonies

<a name="h.k28xj92cimb8"></a>i5-36-2 3:1 → i5-38-3 → 0 colonies

<a name="h.7fp5osalqr18"></a>i5-36-2 0:1 → i5-38-4 → 1 colonies

<a name="h.iwcul570p9na"></a>Control → i5-38-11

<a name="h.a8qnl3t33nlo"></a>

<a name="h.c4b87hf8e4u2"></a>Digestion

<a name="h.chyifkx13l2y"></a>Digested parts

<a name="h.e5i57g1il10m"></a>i5-38-5 (Backbone- CP-Cra) → 19.0

<a name="h.wukqvlly8r0c"></a>i5-38-6 (Backbone- CP-Scr) → 43.2

<a name="h.abr9bnvsvkgm"></a>i5-38-7 (pFru-RBS-GFP) → 11.3

<a name="h.daq45zhhkkz5"></a>i5-38-8 (pScr-RBS-GFP) → 4.9

<a name="h.9rw4l13zbphq"></a>

<a name="h.n2fbxy6ebygf"></a>Ligation

<a name="h.mtbov0uvp4oe"></a>

<a href="#" name="dd5a8cd271d9a457ce7650362cb1f18cd6095058"></a><a href="#" name="61"></a><tbody></tbody>

<a name="h.saafwxx92ife"></a>

<a name="h.zngy8nh8t0c"></a>Insert: i5-38-7 (pFru-RBS-GFP)

<a name="h.b2s1h0zgot4e"></a>Vector: i5-38-5 (Backbone-CP-Cra)

<a name="h.larjp1pa1p5r"></a>

<a name="h.8n8dcb1c3pya"></a>3:1

<a name="h.ila13r6f7fe0"></a>0:1

<a name="h.d6f2eeurl6w5"></a>Insert

<a name="h.p9twdfyqnnr"></a>4.4

<a name="h.cs1kt2qhih0"></a>0

<a name="h.1fnje6wmn90k"></a>Vector

<a name="h.vjl1f549c3b6"></a>2.6

<a name="h.3yy0tohq2vkg"></a>2.6

<a name="h.o986boq4pg56"></a>Buffer

<a name="h.2uxkdcxph2iu"></a>2

<a name="h.xa7jauc842h1"></a>2

<a name="h.a5dgfsjas4of"></a>Ligase

<a name="h.lj6ftrh52v0l"></a>1

<a name="h.bwajompcq8rn"></a>1

<a name="h.ez3nqb2i6p2c"></a>Water

<a name="h.yvi76vssdmla"></a>10

<a name="h.4h7kxerze1n8"></a>14.4

<a name="h.r8lccqmt9kep"></a>Total

<a name="h.fvmmsj4rq1e9"></a>20

<a name="h.s2ijm1ptkp4j"></a>20

<a name="h.ro5oph2r63qm"></a>

<a name="h.86ekxis5zcy4"></a>1

<a name="h.oi3nbmngom5u"></a>2

<a name="h.opedeazge4c3"></a>

<a href="#" name="1f025f77f4d97a10d115c11b553bcfdd96e94039"></a><a href="#" name="62"></a><tbody></tbody>

<a name="h.luz9d9u2roo4"></a>

<a name="h.1mx048cy7a66"></a>Insert: i5-38-8 (pScr-RBS-GFP)

<a name="h.d38pefz839xv"></a>Vector: i5-38-6 (Backbone-CP-SCr)

<a name="h.pz8gd921dj79"></a>

<a name="h.1b0li5mc4w8g"></a>3:1

<a name="h.gu6vcuegktuy"></a>0:1

<a name="h.d6f2eeurl6w5"></a>Insert

<a name="h.mvxyrz3lem07"></a>9.2

<a name="h.xa9jn6dvh9hn"></a>0

<a name="h.1fnje6wmn90k"></a>Vector

<a name="h.hop631q5blju"></a>1.2

<a name="h.sxfooeqscf76"></a>1.2

<a name="h.o986boq4pg56"></a>Buffer

<a name="h.bnpzz3i5811g"></a>2

<a name="h.mgripwfa4eml"></a>2

<a name="h.a5dgfsjas4of"></a>Ligase

<a name="h.lhmoobl0yz5y"></a>1

<a name="h.p261b7hwttvu"></a>1

<a name="h.ez3nqb2i6p2c"></a>Water

<a name="h.om2zvwusi0oj"></a>6.6

<a name="h.bfarl4enbmbv"></a>15.8

<a name="h.r8lccqmt9kep"></a>Total

<a name="h.ub6tthpcn1n9"></a>20

<a name="h.ksg5qvl5c9s7"></a>20

<a name="h.z1but4l66e9c"></a>

<a name="h.c2v6kg515cm9"></a>3

<a name="h.67vm5tw4jb6l"></a>4

<a name="h.m76gb2uljerz"></a>

<a name="h.308r4he0txvj"></a>1→ i5-39-1 (3:1 CP-Cra-pFri-RBS-GFP) → 100+ colonies

<a name="h.ghls9styo5zv"></a>2→ i5-39-2 (0:1)--> 300+ colonies

<a name="h.3ahcudohx7yw"></a>3→ i5-39-3 (3:! CP-Scr-pScr-RBS-GFP) → 200+ colonies

<a name="h.6vruk3cepto2"></a>4→ i5-39-4 (0:1) → 200+ colonies

<a name="h.ue2ilzk7m62u"></a>i5-11-5 → positive control (pLac-GFP) → TNTC

<a name="h.7nbtwclc1mi1"></a>

<a name="h.a33r7clke9l9"></a>8/16

<a name="h.n3hd43aw1rcu"></a>Made glycerol stock

<a name="h.3539oxtcjglw"></a>i5-37-1 (CP weak -GFP)

<a name="h.6kzdjah0vru8"></a>i5-37-3 (CP medium-GFP)

<a name="h.nsiyrfar8tjx"></a>i5-37-5 (CP strong-GFP)