Team:Washington/NANODROP PROTOCOL
From 2013.igem.org
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Nanodrop Protocol
Procedure from: http://openwetware.org/wiki/Nanodrop![Description: https://lh6.googleusercontent.com/2L295ngQHXfpIFEW6YlWYjt_oypvI0ncqtuoJV0ZlwEVhUd6XH2V681iUZTU2FLPrZRc1GnXxJiIxyyKLXgUvsz-uOxwh3JQt0_FkhyoPw6QG9L0oapr-ZCmIgJknb89kQ](file:///C|/Users/Kevin/AppData/Roaming/Adobe/Dreamweaver CS5.5/en_US/OfficeImageTemp/clip_image001.png)
Typical DNA peak as measured by the Nanodrop on PC
- Before starting the software module, clean the sample surfaces with DI water to remove any dried sample that might be present.
- Alternatively, you can clean the sample surfaces with a Kimwipe moistened with 70% ethanol.
- Open the Nanodrop program and the appropriate module (e.g., “Nucleic Acid”).
- Wipe off the top and bottom sensors of the instrument with a Kimwipe. These are just the polished ends of fiber optic cable, so wiping is sufficient to prevent carryover.
- Pipette 1-2 μL of DI water onto the sensor. Bring down the lever arm.
- Follow the onscreen prompts to calibrate.
- Wipe the sensors and pipette on 1-2 μL of the corresponding blank (Buffer EB or whatever solution your prep is in). Bring down the lever arm.
- Follow the onscreen prompts to blank.
- Wipe the sensors and pipette on 1-2 μL of your sample. Bring down the lever arm.
- Click Measure and record the concentration measured.
- For DNA, the peak should be at 260 nm, and as a general rule, the 260/280 ratio should be between 1.8 and 2.0.
- To test multiple samples, just wipe the sensor in between measurements with a Kimwipe. Recalibration or re-blanking is not necessary.
- Clean the sample surfaces once more after you are finished.