Template:Kyoto/Notebook/Aug 10

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Contents

1 Aug 10

Aug 10

Colony PCR

Nakamoto

Sample
8/9 BBa_J44000 -(1)
8/9 BBa_J44000 -(2)
NC

PreDenature	Denature	Annealing	Extension	cycle
94 °C	94 °C	55 °C	68 °C	--
5 min	30 sec	30 sec	30 sec	30 cycles

Sample
8/9 BBa_J23106
8/9 BBa_J23100 -(1)
8/9 BBa_J23100 -(2)
NC

PreDenature	Denature	Annealing	Extension	cycle
94 °C	94 °C	55 °C	68 °C	--
5 min	30 sec	30 sec	1 min	30 cycles

Electrophoresis

Tatsui and Nakamoto

Lane	Sample
	100bp ladder
4	8/9 BBa_J44000 -1
5	8/9 BBa_J44000 -2
6	NC

Lane	Sample
	1kbp ladder
1	8/9 BBa_J23106
2	8/9 BBa_J23100 -1
3	8/9 BBa_J23100 -2
4	NC

Liquid Culture

Nakamoto

Sample	medium
8/9 BBa-J23106	LB(+Amp)
8/9 BBa-J23100-1	LB(+Amp)
8/9 BBa-J23100-2	LB(+Amp)
8/9 BBa-J44000-1	LB(+Amp)
8/9 BBa-J44000-2	LB(+Amp)

LB Medium Plate(+Ampicillin)

Nakamoto & Tatsui

volume	200ml
Bacto T2ypton	2g
Bacto yeast extract	1g
NaCl	1g
0.4M NaOHaq	500µL
Agarose Pouder	2g
Ampicillin	40µL

Transformation

Hirano

Name	Sample	Competent Cells	Total	Plate
tetR	3µL	19µL	22µL	Kan
RBS	3µL	19µL	22µL	Amp
lacZα	3µL	19µL	22µL	CP
lacI	3µL	19µL	22µL	CP
Plac	3µL	19µL	22µL	CP

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