Template:Kyoto/Notebook/Aug 11

From 2013.igem.org

Contents

Aug 11

Miniprep

Nakamoto

DNAconcentration[µg/mL]
8/9 BBa_J44000 -(2)100
8/9 BBa_J23106154
8/9 BBa_J23100144
8/10 T7-His-FT92

Colony PCR

Kojima and Yasuda

Sample
BBa_B0034 -(2)
BBa_B0034 -(1)
BBa_R0011 -(2)
BBa_R0011 -(1)
NC
PreDenatureDenatureAnnealingExtensioncycle
94 °C94 °C55 °C68 °C--
5 min30 s30 s20 s30 cycles
Sample
BBa_D0412 -(1)
BBa_D0412 -(2)
NC
PreDenatureDenatureAnnealingExtensioncycle
94 °C94 °C55 °C68 °C--
5 min30 s30 s1 min 30 s30 cycles

Restriction Enzyme Digestion

Kojima anda Yasuda

8/9 BBa_J44000 2EcoRIPstI10x buffer HMilliQtotal
20.20.216.610
20.2--16.810
2--0.216.810
2----17.010
8/9 BBa_J23106EcoRIPstI10x buffer HMilliQtotal
1.30.20.217.310
1.30.2--17.510
1.3--0.217.510
1.3----17.710
8/9 BBa_J23100 2EcoRIPstI10x buffer HMilliQtotal
1.30.20.217.310
1.30.2--17.510
1.3--0.217.510
1.3----17.710
  • incubated at 37 °C for 1 hour.

Electrophoresis

No name

LaneSampleEnzyme1Enzyme2
1100bp ladder----
28/9 BBa_J44000EcoRIPstI
38/9 BBa_J44000EcoRI--
48/9 BBa_J44000--PstI
58/9 BBa_J44000----
68/9 BBa_J23106EcoRIPstI
78/9 BBa_J23106EcoRI--
88/9 BBa_J23106--PstI
98/9 BBa_J23106----
108/9 BBa_J23100EcoRIPstI
118/9 BBa_J23100EcoRI--
128/9 BBa_J23100--PstI
138/9 BBa_J23100----

Igku Aug11electrophoresis1.jpg

LaneSample
1100bp ladder
2BBa_B0034 (1)
3BBa_B0034 (2)
4BBa_R0011 (1)
5BBa_R0011 (2)
6NC
7100bp ladder

11 electrophoresis2.jpg

LaneSample
11kb ladder
2BBa_P0412 (1)
3BBa_P0412 (2)
4NC

Igku Aug11electrophoresis3.jpg