"
Template:Kyoto/Notebook/Aug 26
From 2013.igem.org
Contents |
Aug 26
Gel Extraction
| Lane | DNA | Enzyme |
|---|---|---|
| 1 | 100bp ladder | - |
| 2 | 8/24 tetR aptamer 12_P | EcoRI & SpeI |
| 4 | 8/24 tetR aptamer 12_1R | EcoRI & SpeI |
| 6 | 8/24 tetR aptamer 12_M | EcoRI & SpeI |
before.jpg)
after.jpg)
| Name | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|
| tetR aptamer 12_P (EcoRI & SpeI) | 2.3 | 0.38 | 0.13 |
| tetR aptamer 12_1R (EcoRI & SpeI) | 12.0 | 0.63 | 1.19 |
| tetR aptamer 12_M (EcoRI & SpeI) | 2.0 | 6.43 | 0.03 |
Ligation
| state | Vector | Inserter | Ligation High ver.2 | ||
|---|---|---|---|---|---|
| experiment | 8/21 DT | 5.0 | 8/26 tetR aptamer 12_1R | 2.0 | 3.5 |
| experiment | 8/21 DT | 5.0 | 8/22 pT181 atteniator | 5.0 | 5.0 |
| experiment | 8/21 DT | 5.0 | 8/22 pT181 antisense | 3.0 | 4.0 |
| experiment | 8/21 DT | 5.0 | 8/22 Spinach | 3.0 | 4.0 |
| experiment | 8/22 Plac | 4.0 | 8/22 pT181 attenuator | 5.0 | 4.5 |
| experiment | 8/22 J23100 | 4.0 | 8/22 pT181 attenuator | 5.0 | 4.5 |
| experiment | 8/22 Plac | 4.0 | 8/22 pT181 antisense | 8.0 | 6.0 |
| experiment | 8/22 J23100 | 4.0 | 8/22 pT181 antisense | 8.0 | 6.0 |
Transformation
| Name | Sample | Competent Cells | Total | Plate |
|---|---|---|---|---|
| 8/26 tetR aptamer 12_1R-DT | 1µL | 10µL | 11µL | CP |
| 8/26 pT181 attenuator-DT | 1µL | 10µL | 11µL | CP |
| 8/26 pT181 antisense-DT | 1µL | 10µL | 11µL | CP |
| 8/26 Spinach-DT | 1µL | 10µL | 11µL | CP |
| 8/26 Plac-pT181 attenuator | 1µL | 10µL | 11µL | CP |
| 8/26 Pcon-pT181 attenuator | 1µL | 10µL | 11µL | Amp |
| 8/26 Plac-pT181 antisense | 1µL | 10µL | 11µL | CP |
| 8/26 Pcon-pT181 antisense | 1µL | 10µL | 11µL | Amp |
| Pλ-RBS-luxI-DT | 1µL | 10µL | 11µ | Amp |
