Z-domain

Main Page

Z-domain

The Z-domain is a IgG binding domain. This sequence has two identical domains and was developed by MG Finn[http://ww2.chemistry.gatech.edu/groups/finn/|] [http://parts.igem.org/Part:BBa_K1156001 BBa_K1156001]

Motivation

Since integrins are sizeable proteins, there was some concern that autotransporters and other display technologies would not be able to handle such a large passenger. The Z-domain provides an alternative strategy for protein expression. The Z-domain is less than 500bp, if needed integrin subunits could be combined with a protein with an affinity for this binding site. Therefore autotransporters would only need to transport the small domain and then the cells could be treated with the integrin fusion proteins.

Design

The sequence was optimized using E. coli codon frequency tables. The part does not include a RBS, promoter, or operator.