Team:BYU Provo/Notebook/Phage Purification/Summerexp/Period1/Exp/7.5 Bacterial Preperation

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| colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Phage Purification July - August Notebook: Experiments'''</font>

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: [[Team:BYU_Provo/Phage_Purification|Overview]]

: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]]

: [[Team:BYU Provo/Notebook/Phage_Purification/Springexp|May-June]]

: [[Team:BYU Provo/Notebook/Phage_Purification/Summerexp|July-August]]

: [[Team:BYU Provo/Notebook/Phage_Purification/Fallexp|September-October]]

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<font size="5"> '''7.5 Bacterial Perperation''' </font>

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'''I) Purpose'''
: Grow bacteria for later use in propagation of phage.

'''II) Expected Outcome'''
: Viable colonies of BL21 and E. coli B.

'''III) Reagants Used'''
: E. coli B and BL21 colonies from plates.
: LB plates (2)


'''IV) Actual Procedure'''

: Streak colonies of bacteria in a 4 quadrant pattern and leave in incubator at 37<sup>◦</sup> overnight. Parafilm and store in fridge. 

'''V) Results'''
: Growth of BL21 and E. coli B. The colonies will remain alive for at least one week.
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