Team:BYU Provo/Notebook/Phage Purification/Summerexp/Period1/Exp/7.5 Bacterial Preperation

Phage Purification July - August Notebook: Experiments

7.5 Bacterial Preparation

I) Purpose

Grow bacteria for later use in propagation of phage.

II) Expected Outcome

Viable colonies of BL21 and E. coli B.

III) Reagants Used

E. coli B and BL21 colonies from plates.

LB plates (2)

IV) Actual Procedure

Streak colonies of bacteria in a 4 quadrant pattern and leave in incubator at 37^◦ overnight. Parafilm and store in fridge.

V) Results

Growth of BL21 and E. coli B. The colonies will remain alive for at least one week.