Template:Kyoto/Notebook/Aug 10

==Aug 10==
===Colony PCR===
<div class="experiment">
<span class="author">Nakamoto</span>
{| class="wikitable"
!Sample
|-
|8/9 BBa_J44000 -(1)
|-
|8/9 BBa_J44000 -(2)
|-
|NC
|}
{| class="wikitable"
!PreDenature||Denature||Annealing||Extension||cycle
|-
|94 &deg;C||94 &deg;C||55 &deg;C||68 &deg;C||--
|-
|5 min||30 sec||30 sec||30 sec||30 cycles
|}
{| class="wikitable"
!Sample
|-
|8/9 BBa_J23106
|-
|8/9 BBa_J23100 -(1)
|-
|8/9 BBa_J23100 -(2)
|-
|NC
|}
{| class="wikitable"
!PreDenature||Denature||Annealing||Extension||cycle
|-
|94 &deg;C||94 &deg;C||55 &deg;C||68 &deg;C||--
|-
|5 min||30 sec||30 sec||1 min||30 cycles
|}
</div>

===Electrophoresis===
<div class="experiment">
<span class="author">Tatsui and Nakamoto</span>
{| class="wikitable"
!Lane||Sample
|-
| ||100bp ladder
|-
|4||8/9 BBa_J44000 -1
|-
|4||8/9 BBa_J44000 -2
|-
|6||NC
|}
[[File:igku_xxxxxx.xxx]]<br>
{| class="wikitable"
!Lane||Sample
|-
| ||1kbp ladder
|-
|1||8/9 BBa_J23106
|-
|2||8/9 BBa_J23100 -1
|-
|3||8/9 BBa_J23100 -2
|-
|4||NC
|}
[[File:Igku Aug10electrophoresis1.jpg]]<br>
</div>

===Liquid Culture===
<div class="experiment">
<span class="author">Nakamoto</span>
{| class="wikitable"
!Sample||medium
|-
|8/9 BBa-J23106||LB(+amp) 
|-
|8/9 BBa-J23100-1||LB(+amp) 
|-
|8/9 BBa-J23100-2||LB(+amp) 
|-
|8/9 BBa-J44000-1||LB(+amp) 
|-
|8/9 BBa-J44000-2||LB(+amp) 
|}
</div>

===LB Medium Plate(+Ampicillin)===
<div class="experiment">
<span class="author">Nakamoto & Tatsui</span>
{| class="wikitable"
!volume||200ml
|-
|Bacto T2ypton || 2g
|-
|Bacto yeast extract ||1g
|-
|NaCl||1g
|-
|0.4M NaOHaq||500&micro;L
|-
|Agarose Pouder||2g　
|-
|Ampicillin||40&micro;L
|}
</div>

===Transformation===
<div class="experiment">
<span class="author">Hirano</span>
{| class="wikitable"
!Name||Sample||Competent Cells||Total||Plate
|-
|tetR||3&micro;L||19&micro;L||22&micro;L||Kan
|-
|RBS||3&micro;L||19&micro;L||22&micro;L||Amp
|-
|lacZ&alpha;||3&micro;L||19&micro;L||22&micro;L||CP
|-
|lacI||3&micro;L||19&micro;L||22&micro;L||CP
|-
|Plac||3&micro;L||19&micro;L||22&micro;L||CP
|}
</div>