Team:BYU Provo/Notebook/Phage Purification/Summerexp/Period1/Exp/7.12 Bacterial Preparation

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| colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Phage Purification July - August Notebook: Experiments'''</font>

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: <u> '''Phage Purification''' </u> </font>

: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]]

: [[Team:BYU Provo/Notebook/Phage_Purification/Springexp|May-June]]

: [[Team:BYU Provo/Notebook/Phage_Purification/Summerexp|July-August]]

: [[Team:BYU Provo/Notebook/Phage_Purification/Fallexp|September-October]]

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<font size="5"> '''7.12 Bacterial Preparation''' </font>

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'''I) Purpose'''
: Grow bacteria for later use in propagation of phage.

'''II) Expected Outcome'''
: Viable colonies of E. coli, both E. coli B and BL21 that can be stored in the fridge for one week.

'''III) Reagants Used'''
: E. coli B and BL21 colonies from plates
: LB plates


'''IV) Actual Procedure'''

: Streak colony of bacteria in a quadrant, standard streak pattern and leave in shaker at 37<sup>◦</sup> overnight. Parafilm and move to fridge.

'''V) Results'''
: Growth of both bacterial types with isolated colonies available. The bacteria will be stored and viable for one week.
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