|
- Phage Purification
- March-April
- May-June
- July-August
- September-October
|
7.12 Bacterial Preparation
I) Purpose
- Grow bacteria for later use in propagation of phage.
II) Expected Outcome
- Viable colonies of E. coli, both E. coli B and BL21 that can be stored in the fridge for one week.
III) Reagants Used
- E. coli B and BL21 colonies from plates
- LB plates
IV) Actual Procedure
- Streak colony of bacteria in a quadrant, standard streak pattern and leave in shaker at 37◦ overnight. Parafilm and move to fridge.
V) Results
- Growth of both bacterial types with isolated colonies available. The bacteria will be stored and viable for one week.
|
"
