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03/09/13
From 2013.igem.org
(Difference between revisions)
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* The pSB1C3 RFP cells that were incubated in luria broth overnight yesterday, were minipreped using the OMEGA bio-tek protocol, in order to isolate plasmid of interest. | * The pSB1C3 RFP cells that were incubated in luria broth overnight yesterday, were minipreped using the OMEGA bio-tek protocol, in order to isolate plasmid of interest. | ||
| - | + | Result of Miniprep Nano Drop | |
{| border=1 | {| border=1 | ||
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|2|| 40|| 62.3|| 1.88|| 2.02 | |2|| 40|| 62.3|| 1.88|| 2.02 | ||
|} | |} | ||
| + | |||
| + | ==Double Digest of the pSB1C3 miniprep== | ||
| + | Samples 1 and 2 were transfered to new eppendorf tubes | ||
| + | *In each tube it was added | ||
| + | **39ul of DNA (miniprep) | ||
| + | **1ul of XbaI | ||
| + | **2ul of SpeI | ||
| + | **6ul of cut smart buffer | ||
| + | **12ul of sterile water | ||
| + | The samples were incubated at 37 degrees water buffer | ||
| + | After the samples were incubated at 80 degrees to kill the enzymes | ||
Revision as of 14:09, 3 September 2013
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Miniprep of cells
- The pSB1C3 RFP cells that were incubated in luria broth overnight yesterday, were minipreped using the OMEGA bio-tek protocol, in order to isolate plasmid of interest.
Result of Miniprep Nano Drop
| Samples | volume (ul) | Concentration (ng/ul) | 260/280 | 260/230 |
| 1 | 40 | 56.2 | 1.89 | 1.95 |
| 2 | 40 | 62.3 | 1.88 | 2.02 |
Double Digest of the pSB1C3 miniprep
Samples 1 and 2 were transfered to new eppendorf tubes
- In each tube it was added
- 39ul of DNA (miniprep)
- 1ul of XbaI
- 2ul of SpeI
- 6ul of cut smart buffer
- 12ul of sterile water
The samples were incubated at 37 degrees water buffer After the samples were incubated at 80 degrees to kill the enzymes
