Exeter/3 September 2013
From 2013.igem.org
(Difference between revisions)
Line 46: | Line 46: | ||
! Part !! Did it work? !! | ! Part !! Did it work? !! | ||
|- | |- | ||
- | | NP1 (CcaS + Terminator) || ✗ | + | | NP1 (CcaS + Terminator) || ✗ | |
|- | |- | ||
- | | NP2 (promoter, RBS, FixJ + Terminator || ✗ | + | | NP2 (promoter, RBS, FixJ + Terminator || ✗ | |
|- | |- | ||
- | | NP3 (yellow pigment + Terminator) || ✗ | + | | NP3 (yellow pigment + Terminator) || ✗ | |
|- | |- | ||
- | | NP4 (magenta pigment + Terminator) || ✗ | + | | NP4 (magenta pigment + Terminator) || ✗ | |
|- | |- | ||
- | | NP7 (YF1 + Terminator) || ✓ | + | | NP7 (YF1 + Terminator) || ✓ | |
|- | |- | ||
- | | NP8 (FixJ + Terminator) || ✓ | + | | NP8 (FixJ + Terminator) || ✓ | |
|- | |- | ||
- | | Ligation control || ✗ | + | | Ligation control || ✗ | |
|- | |- | ||
- | | Lambda inverter system || ✗ | + | | Lambda inverter system || ✗ | |
|- | |- | ||
| T7 promoter || ✗ || | | T7 promoter || ✗ || | ||
|- | |- | ||
- | | Promoter + RBS (BBa_K608002) || ✓ | + | | Promoter + RBS (BBa_K608002) || ✓ | |
|- | |- | ||
- | | RBS (BBa_B0034) || ✓ | + | | RBS (BBa_B0034) || ✓ | |
|- | |- | ||
- | | OmpR "A" || ✓ | + | | OmpR "A" || ✓ | |
|- | |- | ||
- | | OmpR "B" || ✗ | + | | OmpR "B" || ✗ | |
|- | |- | ||
- | | RFP control || ✓ | + | | RFP control || ✓ | |
|- | |- | ||
- | | RBS + cph8 (BBa_K592018) || ✓ | + | | RBS + cph8 (BBa_K592018) || ✓ | |
|} | |} |
Revision as of 14:30, 3 September 2013
Results of liquid cultures from yesterday
gBlocks
gBlock | Colour |
---|---|
1 | No |
2 | No |
3 | Vibrant pink |
4 | No |
5 | Vibrant pink |
6 | No |
7 | Peach |
8 | No |
9 | Pink - not so vibrant |
10 | Vibrant pink |
11 | Vibrant pink |
12 | Pink - not so vibrant |
13 | Pink - not so vibrant |
14 | No |
15 | No |
Part | Did it work? | |
---|---|---|
NP1 (CcaS + Terminator) | ||
NP2 (promoter, RBS, FixJ + Terminator | ||
NP3 (yellow pigment + Terminator) | ||
NP4 (magenta pigment + Terminator) | ||
NP7 (YF1 + Terminator) | ||
NP8 (FixJ + Terminator) | ||
Ligation control | ||
Lambda inverter system | ||
T7 promoter | ✗ | |
Promoter + RBS (BBa_K608002) | ||
RBS (BBa_B0034) | ||
OmpR "A" | ||
OmpR "B" | ||
RFP control | ||
RBS + cph8 (BBa_K592018) |