Exeter/3 September 2013
From 2013.igem.org
(Difference between revisions)
(→Miniprepped) |
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Line 75: | Line 75: | ||
*K592011 B | *K592011 B | ||
*gblocks 1 to 15 | *gblocks 1 to 15 | ||
+ | |||
+ | == NanoDrop from miniprep == | ||
+ | |||
+ | {| class="wikitable" | ||
+ | |- | ||
+ | ! Part !! Concentration (ng/ul) | ||
+ | |- | ||
+ | | CcaR ||324.6 | ||
+ | |- | ||
+ | | CcaS || 264.4 | ||
+ | |- | ||
+ | | Yellow || 64.3 | ||
+ | |- | ||
+ | | Magenta || 102.7 | ||
+ | |- | ||
+ | | RBS || 48.9 | ||
+ | |- | ||
+ | | Promoter, RBS, #1 || 21.5 | ||
+ | |- | ||
+ | | Promoter, RBS, #2 || 22.6 | ||
+ | |- | ||
+ | | Promoter, RBS, #3 || 22.0 | ||
+ | |- | ||
+ | | Fix L || 31.9 | ||
+ | |- | ||
+ | | Fix J || 35.4 | ||
+ | |- | ||
+ | | YF1 || 27.1 | ||
+ | |- | ||
+ | | OmpF || 28.2 | ||
+ | |} |
Revision as of 14:43, 3 September 2013
Contents |
Results of liquid cultures from yesterday
gBlocks
gBlock | Colour |
---|---|
1 | No |
2 | No |
3 | Vibrant pink |
4 | No |
5 | Vibrant pink |
6 | No |
7 | Peach |
8 | No |
9 | Pink - not so vibrant |
10 | Vibrant pink |
11 | Vibrant pink |
12 | Pink - not so vibrant |
13 | Pink - not so vibrant |
14 | No |
15 | No |
Part | Did it work? |
---|---|
OmpF + RBS A | ✓ |
OmpF + RBS B | ✓ |
OmpF + RBS C | ✓ |
OmpF + RBS D | ✓ |
K592022 A | ✗ |
K592022 B | ✓ |
K592011 A | ✗ |
K592011 B | ✓ |
K864404 | ✓ |
Miniprepped
- OmpF + RBS A
- OmpF + RBS B
- OmpF + RBS C
- OmpF + RBS D
- K592011 B
- gblocks 1 to 15
NanoDrop from miniprep
Part | Concentration (ng/ul) |
---|---|
CcaR | 324.6 |
CcaS | 264.4 |
Yellow | 64.3 |
Magenta | 102.7 |
RBS | 48.9 |
Promoter, RBS, #1 | 21.5 |
Promoter, RBS, #2 | 22.6 |
Promoter, RBS, #3 | 22.0 |
Fix L | 31.9 |
Fix J | 35.4 |
YF1 | 27.1 |
OmpF | 28.2 |