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Team:BYU Provo/Notebook/LargePhage/Summerexp/Period1/Dailylog
From 2013.igem.org
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| + | In general, the plaques for the mutagenized phage seem to be smaller than the pre-mutagenized phage. From this it would appear that we have large phage! But, it's important to make sure that we have a stable phage, so from here we will be picking the small plaques from the mutant plate and plating them out so we can observe if there is a steady result of small plaques. | ||
===9/9/13=== | ===9/9/13=== | ||
Revision as of 20:30, 6 September 2013
Contents |
September
9/4/13
Results from 8/28/13: Fractions 20 and 21 produced clearage on 10^0, and many small plaques at 10^-2. #4 had one plaque on 10^-2, and #1 had one plaque on 10^0.
Today we infected 0.5 mL of E. coli with: 100 uL of #1 10^0, 100 uL of #4 10^0, 10 uL of #20 10^-2, 10 uL of #21 10^-2, and for controls we did 10 uL of T4 wild type 10^-6 and 1 uL of T4 10^-7. We also plated a lawn of E. coli as a control.
9/6/13
Results from 9/2:
In general, the plaques for the mutagenized phage seem to be smaller than the pre-mutagenized phage. From this it would appear that we have large phage! But, it's important to make sure that we have a stable phage, so from here we will be picking the small plaques from the mutant plate and plating them out so we can observe if there is a steady result of small plaques.
9/9/13
9/11/13
9/13/13
