Team:BYU Provo/Notebook/CholeraDetection/Springexp/Period1/Dailylog

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Our results from the side-by-side plate comparison of grown cholera with several strains of bacteria are encouraging!  We grew V. cholerae from one week
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Clarice transformed the iGEM backbone into E.Coli and grew it up in an overnight. From those cells we isolated the plasmid, pIG91, in two Eppendorfs, at concentrations of 52 ng/uL and 82 ng/uL. Monday we will digest the plasmid and the SdiA gene to splice it in and submit to the registry.
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KK, CH, KP
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State of Affairs:
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Regarding CRO control of lysis in our E.Coli hosts:
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CRO is inserted into pIG12 and is sequence verified. PIG12 was electroporated into TT9901 and TT9907, which we have verified have the dormant Lambda prophage in their genomes. The electroporation into TT25281 failed. We are plating out TT25281 fresh from a stab, and will electroporate it again. Today, we plated patches of TT9901 and T9907 on LB+amp+arabinose!
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Regarding Quorum Sensing
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PCR products of HapA promotor region and CQSS looked good on the gel. We need to redo GFP.
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Revision as of 01:25, 7 September 2013


Cholera Detection May - June Notebook: July 1 - July 7 Daily Log



Overview
March-April
May-June
July-August
September-October

9/4/2013



9/6/13 Our results from the side-by-side plate comparison of grown cholera with several strains of bacteria are encouraging! We grew V. cholerae from one week

Clarice transformed the iGEM backbone into E.Coli and grew it up in an overnight. From those cells we isolated the plasmid, pIG91, in two Eppendorfs, at concentrations of 52 ng/uL and 82 ng/uL. Monday we will digest the plasmid and the SdiA gene to splice it in and submit to the registry.

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