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Team:UNITN-Trento/Notebook/Labposts/06/43
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(Created page with "{ "date" : "2013-06-22", "author" : "bruno", "title" : "Minipreps and screening of Promoters", "content" : "<html>From inocula of the day before I made the minipreps by the f...") |
Revision as of 07:44, 9 September 2013
{ "date" : "2013-06-22", "author" : "bruno", "title" : "Minipreps and screening of Promoters", "content" : "From inocula of the day before I made the minipreps by the followingprotocol.
Quantification results
I made the screening test. To do that we digested 1500 ng of DNA with EcorI and PstI following the screening digestion protocol. | Sample | ng/ul |
|---|---|
| K090501 | 117,1 |
| K090504_a | 115,3 |
| K090504_b | 101,3 |
| K823000_a | 156,0 |
| K823000_b | 171,1 |
| K823000 | 115,5 |
I used a 1.5% concentration of agarose to create a gel and I used Ethidium bromide instead the normal EuroSafe. In addition I have not used a normal Dye with the 20ul of sample loaded but I used 4ul of 30% glycerol. ", "tags" : "lacOid-PliaG-Pgram+" }
