Team:UNITN-Trento/Notebook/Labposts/07/53
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(Created page with "{ "date" : "2013-07-10", "author" : "emil", "title" : " Purification of Inocula(09/7): the final countdown", "content" : "<html>I (yes I'm egocentric) did the miniprep(wath a...") |
Revision as of 07:53, 9 September 2013
{ "date" : "2013-07-10", "author" : "emil", "title" : " Purification of Inocula(09/7): the final countdown", "content" : "I (yes I'm egocentric) did the miniprep(wath a new thing!) of the inocula(I didn't purified the 1:2 due to their despicable red color) previously done(the last I hope) following(is always the same but I have to write it anyway)the miniprep protocol(OOOH!that's incredible!).These are(obviously)the results of the quantification:
results
Afterwards I screened them(800 ng for each sample) following the digestion protocol so I have obtained these results:Sample | Quantities |
---|---|
BBa_K823026+BBa_E0840(1:1) | 421.3ng/µl |
BBa_K823026+BBa_E0840(1:1) | 438.6ng/µl | BBa_K823026+BBa_E0840(1:3) | 356.7ng/µl |
BBa_K823026+BBa_E0840(1:3) | 415.2ng/µl |
BBa_K823024+BBa_E0840 | 401.2ng/µl | BBa_K823024+BBa_E0840 | 365ng/µl |
gel order
As we can see in the last 4 samples the lower band is clearly at 1000 bp then the GFP is probably present, the succesfull samples were sent to be sequenced!",
"tags" : "pSpac-PXyl-GFP"
}
Sample | Well |
---|---|
ladder | 1 |
BBa_K823026+BBa_E0840(1:1) | 2 |
BBa_K823026+BBa_E0840(1:1) | 3 | BBa_K823026+BBa_E0840(1:3) | 4 |
BBa_K823026+BBa_E0840(1:3) | 5 |
BBa_K823024+BBa_E0840 | 6 | BBa_K823024+BBa_E0840 | 7 |