Team:BYU Provo/Notebook/SmallPhage/Winterexp/Period3/Dailylog

Small Phage

March-April

May-June

July-August

September-October

Presentation

4/3/13

- Learned about how to start an overnight liquid culture for host bacteria

- Learned about how to create bacterial glycerol stock

- Made fresh LB and concentrated top agar stock

4.3 Top Agar Stock Preparation

4/4/13

- T7 arrived

- Overnight liquid culture for bacterial host started by Dr. Grose

4/5/13

- Performed spot test with E coli BL21 overnight liquid culture and WT T7 phage

4.5 T7 phage viability assay

- Created bacterial glycerol stock

0.5mL of bacteria overnight liquid culture + 0.5mL of glycerol

4/6/13

- Checked up on 4.5 T7 phage viability assay

- 6:00pm overnight E coli liquid culture

W3110 one colony in 25mL Erlenmeyer flask

BL21 one stab in 25mL Erlenmeyer flask

- 9:00pm Streak bacteria culture to check for contamination and for storage

performed for both W3110 and BL21

4/8/13

- Performed 4.8 T7 phage viability assay 2

- Outlined final presentation content and divided up assignments

4/9/13

- Checked up on 4.8 T7 phage viability assay 2

- Start overnight for E coli BL21

4/10/13

- Performed 4.10 T7 phage viability assay 3

- Looked into T7-family phage

4/11/13

- Check up on 4.10 T7 phage viability assay 3

- Worked on Presentation

4/12/13

- Cholera Presentation

- Worked on Presentation

4/13/13

- Continued working on Presentation