Team:BYU Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU Provo/5.5 Amplification from a plaque test
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- | ===5.5 Amplification from a plaque test | + | {{TeamBYUProvo}} |
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+ | | colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Small Phage May - June Notebook: Experiments'''</font> | ||
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+ | : <u> '''Small Phage''' </u> </font> | ||
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+ | : [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]] | ||
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+ | : [[Team:BYU Provo/Notebook/SmallPhage/Springexp|May-June]] | ||
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+ | : [[Team:BYU Provo/Notebook/SmallPhage/Summerexp|July-August]] | ||
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+ | : [[Team:BYU Provo/Notebook/SmallPhage/Fallexp|September-October]] | ||
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+ | </font> | ||
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+ | | style="width: 82%; background-color: transparent;"| | ||
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+ | <font face="Calibri" size="3"> | ||
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+ | <font size="5"> '''5.5 Amplification from a plaque test''' </font> | ||
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'''I) Purpose''' | '''I) Purpose''' | ||
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'''III) Reagants Used''' | '''III) Reagants Used''' | ||
- | : E. Coli BL21 overnight from 5.4; ×6 plate from 5.3 | + | : E. Coli BL21 overnight from 5.4; ×6 plate from [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU_Provo/5.3 Phage Amplification/Purification|5.3 T7 phage selection method test]]; LB from 4.3 |
'''IV) Actual Procedure''' | '''IV) Actual Procedure''' | ||
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1. Amplifying phage from a single plaque (5.5) | 1. Amplifying phage from a single plaque (5.5) | ||
: To a test tube, add 1mL of E coli BL21 overnight (5.4) and 4mL of LB | : To a test tube, add 1mL of E coli BL21 overnight (5.4) and 4mL of LB | ||
- | : Stab one of the plaques on a ×6 plate from the 5.3 | + | : Stab one of the plaques on a ×6 plate from the [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU_Provo/5.3 Phage Amplification/Purification|5.3 T7 phage selection method test]] with a 1mL pipet tip, puncturing the agar. The plaque in question is labeled with a circle. |
: The pipet tip is then dipped into the overnight/LB solution. The solution is then pipetted up and down several times to maximize the amount of phage that gets into the solution. | : The pipet tip is then dipped into the overnight/LB solution. The solution is then pipetted up and down several times to maximize the amount of phage that gets into the solution. | ||
: The solution was then incubated for approximately 24 hours (5.5 noon - 5.6 2:00pm ) | : The solution was then incubated for approximately 24 hours (5.5 noon - 5.6 2:00pm ) | ||
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2. Spot test to test amplification results (5.6) | 2. Spot test to test amplification results (5.6) | ||
- | : Phage liquid culture was purified and spot test was performed using procedures similar to those in [[5.3 Phage Amplification/Purification]]. Stock created was labeled 5.4 | + | : Phage liquid culture was purified and spot test was performed using procedures similar to those in [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU_Provo/5.3 Phage Amplification/Purification|5.3 T7 phage amplification/purification]]. Stock created was labeled 5.4 |
: Spot test plates were incubated overnight (5.6 4:00pm - 5.7 12:00pm ) | : Spot test plates were incubated overnight (5.6 4:00pm - 5.7 12:00pm ) | ||
'''V) Results (5.7)''' | '''V) Results (5.7)''' | ||
: Our method of amplification from a single plaque was successful. Phage plaque was observed up to -7 | : Our method of amplification from a single plaque was successful. Phage plaque was observed up to -7 | ||
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+ | : [[File:5.4 stock spot test.JPG|600px|left]] | ||
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+ | </font> | ||
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+ | |} | ||
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+ | {{TeamBYUProvoFooter}} |
Latest revision as of 13:34, 9 September 2013
Small Phage May - June Notebook: Experiments
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5.5 Amplification from a plaque test
II) Expected Outcome
III) Reagants Used
IV) Actual Procedure 1. Amplifying phage from a single plaque (5.5)
2. Spot test to test amplification results (5.6)
V) Results (5.7)
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