Team:BYU Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU Provo/5.6 T7+ Liquid Culture Phage Concentration Test
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- | ===5.6 T7+ Liquid Culture Phage Concentration Test | + | {{TeamBYUProvo}} |
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+ | | colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Small Phage May - June Notebook: Experiments'''</font> | ||
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+ | : <u> '''Small Phage''' </u> </font> | ||
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+ | : [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]] | ||
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+ | : [[Team:BYU Provo/Notebook/SmallPhage/Springexp|May-June]] | ||
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+ | : [[Team:BYU Provo/Notebook/SmallPhage/Summerexp|July-August]] | ||
+ | |||
+ | : [[Team:BYU Provo/Notebook/SmallPhage/Fallexp|September-October]] | ||
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+ | </font> | ||
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+ | | style="width: 82%; background-color: transparent;"| | ||
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+ | <font face="Calibri" size="3"> | ||
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+ | <font size="5"> '''5.6 T7+ Liquid Culture Phage Concentration Test''' </font> | ||
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'''I) Purpose''' | '''I) Purpose''' | ||
- | To determine if the volume of phage stock added effects the liquid culture concentration result | + | : To determine if the volume of phage stock added effects the liquid culture concentration result |
'''II) Expected Outcome''' | '''II) Expected Outcome''' | ||
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'''III) Reagants Used''' | '''III) Reagants Used''' | ||
- | : E. Coli BL21 overnight from 5.5; T7+ from 5.3 Phage Amplification/Purification | + | : E. Coli BL21 overnight from 5.5; T7+ from [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU_Provo/5.3 Phage Amplification/Purification|5.3 T7 phage amplification/purification]]; LB from 4.5 |
'''IV) Actual Procedure''' | '''IV) Actual Procedure''' | ||
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Liquid culture set up (5.6) | Liquid culture set up (5.6) | ||
: - Three test tubes were set up. To each, add 4mL of LB and 1mL of E coli BL21 overnight | : - Three test tubes were set up. To each, add 4mL of LB and 1mL of E coli BL21 overnight | ||
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: - Liquid culture was incubated for approximately 48 hours (5.6 4:00pm - 5.8 6:00pm) | : - Liquid culture was incubated for approximately 48 hours (5.6 4:00pm - 5.8 6:00pm) | ||
Spot test to test amplification results (5.8) | Spot test to test amplification results (5.8) | ||
- | : - Phage liquid culture was purified and spot test was performed using procedures similar to those in 5.3 Phage Amplification/Purification | + | : - Phage liquid culture was purified and spot test was performed using procedures similar to those in [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU_Provo/5.3 Phage Amplification/Purification|5.3 T7 phage amplification/purification]] |
: - The purified stock was labeled 5.6 | : - The purified stock was labeled 5.6 | ||
: - Spot test plates were incubated overnight | : - Spot test plates were incubated overnight | ||
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: - The 10 ul spot test went down to -9, 100 ul to -8, and 1 ml to -7 | : - The 10 ul spot test went down to -9, 100 ul to -8, and 1 ml to -7 | ||
: - Thus, 10 ul yields the highest titer | : - Thus, 10 ul yields the highest titer | ||
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+ | : [[File:5.10 phage concentration test.JPG|600px|left]] | ||
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+ | </font> | ||
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+ | |} | ||
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+ | {{TeamBYUProvoFooter}} |
Latest revision as of 13:35, 9 September 2013
Small Phage May - June Notebook: Experiments
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5.6 T7+ Liquid Culture Phage Concentration Test
II) Expected Outcome
III) Reagants Used
IV) Actual Procedure Liquid culture set up (5.6)
Spot test to test amplification results (5.8)
V) Results (5.9)
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