Team:BYU Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU Provo/5.6 T7+ Liquid Culture Phage Concentration Test

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===5.6 T7+ Liquid Culture Phage Concentration Test===
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| colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Small Phage May - June Notebook: Experiments'''</font>
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: <u> '''Small Phage''' </u> </font>
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: [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]]
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: [[Team:BYU Provo/Notebook/SmallPhage/Springexp|May-June]]
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: [[Team:BYU Provo/Notebook/SmallPhage/Summerexp|July-August]]
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: [[Team:BYU Provo/Notebook/SmallPhage/Fallexp|September-October]]
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<font size="5"> '''5.6 T7+ Liquid Culture Phage Concentration Test''' </font>
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'''I) Purpose'''
'''I) Purpose'''
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To determine if the volume of phage stock added effects the liquid culture concentration result
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: To determine if the volume of phage stock added effects the liquid culture concentration result
'''II) Expected Outcome'''
'''II) Expected Outcome'''
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'''III) Reagants Used'''
'''III) Reagants Used'''
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: E. Coli BL21 overnight from 5.5; T7+ from 5.3 Phage Amplification/Purification test; LB from 4.5
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: E. Coli BL21 overnight from 5.5; T7+ from [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU_Provo/5.3 Phage Amplification/Purification|5.3 T7 phage amplification/purification]]; LB from 4.5
'''IV) Actual Procedure'''
'''IV) Actual Procedure'''
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Liquid culture set up (5.6)
Liquid culture set up (5.6)
: - Three test tubes were set up. To each, add 4mL of LB and 1mL of E coli BL21 overnight  
: - Three test tubes were set up. To each, add 4mL of LB and 1mL of E coli BL21 overnight  
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: - Liquid culture was incubated for approximately 48 hours (5.6 4:00pm - 5.8 6:00pm)
: - Liquid culture was incubated for approximately 48 hours (5.6 4:00pm - 5.8 6:00pm)
Spot test to test amplification results (5.8)
Spot test to test amplification results (5.8)
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: - Phage liquid culture was purified and spot test was performed using procedures similar to those in 5.3 Phage Amplification/Purification
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: - Phage liquid culture was purified and spot test was performed using procedures similar to those in [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU_Provo/5.3 Phage Amplification/Purification|5.3 T7 phage amplification/purification]]
: - The purified stock was labeled 5.6
: - The purified stock was labeled 5.6
: - Spot test plates were incubated overnight
: - Spot test plates were incubated overnight
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: - The 10 ul spot test went down to -9, 100 ul to -8, and 1 ml to -7
: - The 10 ul spot test went down to -9, 100 ul to -8, and 1 ml to -7
: - Thus, 10 ul yields the highest titer
: - Thus, 10 ul yields the highest titer
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: [[File:5.10 phage concentration test.JPG|600px|left]]
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Latest revision as of 13:35, 9 September 2013


Small Phage May - June Notebook: Experiments



Small Phage
March-April
May-June
July-August
September-October

5.6 T7+ Liquid Culture Phage Concentration Test



I) Purpose

To determine if the volume of phage stock added effects the liquid culture concentration result

II) Expected Outcome

We hope that 100uL of phage will be enough to increase the liquid culture concentration

III) Reagants Used

E. Coli BL21 overnight from 5.5; T7+ from 5.3 T7 phage amplification/purification; LB from 4.5

IV) Actual Procedure

Liquid culture set up (5.6)

- Three test tubes were set up. To each, add 4mL of LB and 1mL of E coli BL21 overnight
- Add 10uL, 100uL, and 1mL to each tube, respectively.
- Liquid culture was incubated for approximately 48 hours (5.6 4:00pm - 5.8 6:00pm)

Spot test to test amplification results (5.8)

- Phage liquid culture was purified and spot test was performed using procedures similar to those in 5.3 T7 phage amplification/purification
- The purified stock was labeled 5.6
- Spot test plates were incubated overnight

V) Results (5.9)

- The 10 ul spot test went down to -9, 100 ul to -8, and 1 ml to -7
- Thus, 10 ul yields the highest titer
5.10 phage concentration test.JPG


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