Team:BYU Provo/Notebook/SmallPhage/Springexp/Period1/Exp/5.9 T7+ Liquid Culture Phage Concentration Test
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- | ===5.9 T7+ Liquid Culture Phage Concentration Test #2 | + | <br> |
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+ | {| width="100%" | ||
+ | | colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Small Phage May - June Notebook: Experiments'''</font> | ||
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+ | <br> | ||
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+ | | style="width: 18%; background-color: transparent;"| | ||
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+ | : <u> '''Small Phage''' </u> </font> | ||
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+ | : [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]] | ||
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+ | : [[Team:BYU Provo/Notebook/SmallPhage/Springexp|May-June]] | ||
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+ | : [[Team:BYU Provo/Notebook/SmallPhage/Summerexp|July-August]] | ||
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+ | : [[Team:BYU Provo/Notebook/SmallPhage/Fallexp|September-October]] | ||
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+ | </font> | ||
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+ | | style="width: 82%; background-color: transparent;"| | ||
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+ | <font face="Calibri" size="3"> | ||
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+ | <font size="5"> '''5.9 T7+ Liquid Culture Phage Concentration Test #2''' </font> | ||
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+ | <br> | ||
'''I) Purpose''' | '''I) Purpose''' | ||
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2. Spot test to test amplification results | 2. Spot test to test amplification results | ||
- | : Phage liquid culture was purified using procedures similar to those in [[5.3 T7 phage amplification/purification]]. The resulted purified stock was labeled 5.13 (5.13) | + | : Phage liquid culture was purified using procedures similar to those in [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU_Provo/5.3 Phage Amplification/Purification|5.3 T7 phage amplification/purification]]. The resulted purified stock was labeled 5.13 (5.13) |
- | : Spot test was performed using procedures similar to those in [[5.3 T7 phage amplification/purification]] (5.15) | + | : Spot test was performed using procedures similar to those in [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU_Provo/5.3 Phage Amplification/Purification|5.3 T7 phage amplification/purification]] (5.15) |
: Spot test plates were incubated overnight | : Spot test plates were incubated overnight | ||
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: Complete clearage of liquid culture was observed for once | : Complete clearage of liquid culture was observed for once | ||
: All spot tests showed plaques to -8, but there was also a lot of contamination -> from the LB plates; the plates have been completely disposed of. | : All spot tests showed plaques to -8, but there was also a lot of contamination -> from the LB plates; the plates have been completely disposed of. | ||
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+ | |} | ||
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Latest revision as of 13:35, 9 September 2013
Small Phage May - June Notebook: Experiments
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5.9 T7+ Liquid Culture Phage Concentration Test #2
I) Purpose
II) Expected Outcome
III) Reagants Used
IV) Actual Procedure 1. Liquid culture set up (5.9)
2. Spot test to test amplification results
V) Results
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