Team:BYU Provo/Notebook/SmallPhage/Springexp/Period2/Exp/5.20 Mutagen Concentration Experiment
From 2013.igem.org
(2 intermediate revisions not shown) | |||
Line 14: | Line 14: | ||
<font color="#333399" size="3" font face="Calibri"> | <font color="#333399" size="3" font face="Calibri"> | ||
- | : | + | <font size = "4"> |
+ | |||
+ | : <u> '''Small Phage''' </u> </font> | ||
: [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]] | : [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]] | ||
Line 127: | Line 129: | ||
: -6 at x6 only showed a couple plaques on each plate. For -4 at x6, there were many plaques. It seems like the number of plaques increase as we increase the mutagen concentration. | : -6 at x6 only showed a couple plaques on each plate. For -4 at x6, there were many plaques. It seems like the number of plaques increase as we increase the mutagen concentration. | ||
+ | |||
+ | [[File:MutagenPlate4.JPG|400px|center]] | ||
5) Preliminary selection test - third round | 5) Preliminary selection test - third round | ||
Line 135: | Line 139: | ||
: We saw a few bigger plaques (compared to the average plaque sizes) from the 45 plates. These are ones we are interested in. | : We saw a few bigger plaques (compared to the average plaque sizes) from the 45 plates. These are ones we are interested in. | ||
+ | |||
+ | [[File:MutagenPlate5.JPG|400px|center]] | ||
7) Large plaque confirmation | 7) Large plaque confirmation | ||
: Large scale contamination prevented any plaque from showing up. | : Large scale contamination prevented any plaque from showing up. | ||
- | |||
- | |||
'''VI) Conclusion''' | '''VI) Conclusion''' | ||
- | + | Because there appears to be no visible decrease in the number of plaques as the concentration of mutagen increases, mutagenesis probably didn't occur effectively. In order to hopefully fix this, we will try mutagenesis again with a minimal media instead of LB. (See [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/6.12 Mutagen Concentration Test - Perfected Protocol|6.12 Mutagen Concentration Test - Perfected Protocol]]) | |
Latest revision as of 13:38, 9 September 2013
Small Phage May - June Notebook: Experiments
| ||
|
5.20 Mutagen Concentration Experiment
I) Purpose
II) Expected Outcome
III) Reagents Used
IV) Procedure 1) Applying the mutagen (5.20)
2) Spot test (5.22)
3) Preliminary selection test (5.24)
4) Preliminary selection test - second round (5.29)
5) Preliminary selection test - third round (6.3)
6) Selection - round 1 (6.7)
7) Large plaque confirmation (6.10)
V) Results 1) Applying mutagen
2) Spot test
3) Preliminary selection test
4) Preliminary selection test 2
5) Preliminary selection test - third round
6) Selection - round 1
7) Large plaque confirmation
Because there appears to be no visible decrease in the number of plaques as the concentration of mutagen increases, mutagenesis probably didn't occur effectively. In order to hopefully fix this, we will try mutagenesis again with a minimal media instead of LB. (See 6.12 Mutagen Concentration Test - Perfected Protocol)
|