09/09/13

(Difference between revisions)

Revision as of 16:42, 9 September 2013

Home	Team	Official Team Profile	Project	Parts Submitted to the Registry	Modeling	Notebook	Safety	Attributions

Following on from 06/09/13 result,overnight broth was prepared yesterday from the single colonies that had grown on each of the 2 plates shown on 06/09/13.
The broth, which contains cells that have been transformed with pSB1C3/TOD genes, was minipreped using the Omega Bio-Tek kit.
This would enable us to confirm which cells have the recircularised plasmid and those with the ligated product.

The pSB1C3/TOD genes were double digested with (XbaI and SpeI) & (EcoRI and PstI)
The master mix for the (XbaI and SpeI) was;

60ul of cut smart buffer 5ul of XbaI 10ul of SpeI 273ul of 5M Tris

60ul of cut smart buffer 5ul of XbaI 5ul of SpeI 278ul of 5M Tris

All 36 tubes were incubated overnight in 37 degrees room.

Tomorrow the plasmids that were isolated from the miniprep would be run on a 1% agarose gel to distinguish between cells that have the pSB1C3/TOD genes from those that have the recircularised pSB1C3 vector.

@@ Line 33: / Line 33: @@
 |-
 |TOD X6||31.7||70.5||1.88||1.88
+|-
+|
 |-
 |TOD F1||32.4||39.6||1.91||1.59
@@ Line 45: / Line 47: @@
 |-
 |TOD F6||38||59.4||1.89||1.61
+|-
+|
+|-
+|TOB G1||36.8||72.5||1.89||1.51
+|-
+|TOB G2||44.7||1.90||1.90||1.65
+|-
+|TOB G3||33.7||50.2||1.90||1.46
+|-
+|TOB G4||41.9||55||1.93||1.35
+|-
+|TOB G5||34||43||1.96||1.54
+|-
+|TOB G6||35||48||1.89||1.72
 |}