Team:Braunschweig/Notebook

From 2013.igem.org

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     <h2><a href="#Week3">Week 3: June 2 - June 8, 2013</a></h2>
     <h2><a href="#Week3">Week 3: June 2 - June 8, 2013</a></h2>
     <p><p style=" margin-left:5px; margin-right:5px;">This week we successfully cloned and transformed some of our first combination Bricks. We also managed to obtain some BioBricks that could not be transformed via Phusion-PCR directly from resuspended DNA.</p>
     <p><p style=" margin-left:5px; margin-right:5px;">This week we successfully cloned and transformed some of our first combination Bricks. We also managed to obtain some BioBricks that could not be transformed via Phusion-PCR directly from resuspended DNA.</p>
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<p><p style="font-size:14px; font-weight:bold; text-decoration:none; border:none; color:#be1e3c; margin-left:5px; margin-right:5px; margin-top:0px; margin-bottom:0px">Sunday, June 2, 2013</p>
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<p><p style="margin-left:5px; margin-right:5px; margin-bottom:0px; margin-top:0px">
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<b>Investigators: Roman</b><br>
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Since our transformations for the Bricks B0010, C0060, C0061, C0062, C0070, J23100 and J23106 were not successful, they amplified via Phusion-PCR directly from the resuspended DNA from the Distribution Kit.<br>
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We prepared 5 mL liquid cultures of Bricks B0012, B0032, B1009, C0060, C0061, C0062, C0070, C0071, C0078, C0079, E0240, E0430, J06702, R0062, R0071 in order to miniprep them the next day.</p>
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<p><p style="font-size:14px; font-weight:bold; text-decoration:none; border:none; color:#be1e3c; margin-left:5px; margin-right:5px; margin-top:0px; margin-bottom:0px">Monday, June 3, 2013</p>
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<p><p style="margin-left:5px; margin-right:5px; margin-bottom:0px; margin-top:0px">
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<b>Investigators: Oliver, Kerstin, Laura</b><br>
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<img alt="June3" src="https://static.igem.org/mediawiki/2013/d/d4/Braunschweig_Lab_Journal_June_3.png" width="250" align="right" vspace="0" hspace="20"/>We miniprepped the Bricks B0012, B0032, B1009, C0060, C0061, C0062, C0070, C0071, C0078, C0079, E0240, E0430, J06702, R0062 and R0071. As stated earlier the Bricks C0061 and C0062 showed no visible bands in the colony PCR but still were prepped. Furthermore we prepared glycerol stocks of all strains for further use.<br>
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Our gel electrophoresis of the PCR-amplified Bricks showed a suitable bands for BioBricks C0060, C0061, C0070, J23100, J23106 which were recovered successfully from the gel. The Bricks B0010, C0062 showed no bands on the gel and therefore could not be isolated.
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Revision as of 00:11, 10 September 2013

Labjournal

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This is the documentation of our lab work. For detailed protocols of certain procedures please refer to Protocols. Attributions are given for each day, however please check our Attributions section for efforts beyond the lab work.

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