Team:Braunschweig/Notebook
From 2013.igem.org
(Difference between revisions)
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<p><p style="margin-left:5px; margin-right:5px; margin-bottom:0px; margin-top:0px"> | <p><p style="margin-left:5px; margin-right:5px; margin-bottom:0px; margin-top:0px"> | ||
<b>Investigators: Tabea, Jan, Laura</b><br> | <b>Investigators: Tabea, Jan, Laura</b><br> | ||
- | |||
The distribution kit arrived!<br> | The distribution kit arrived!<br> | ||
We resuspended the DNA of 19 BioBricks, measured the DNA concentrations via NanoDrop and did transformed each BioBrick. This is the full list of BioBricks we plan on using in our project:<br> | We resuspended the DNA of 19 BioBricks, measured the DNA concentrations via NanoDrop and did transformed each BioBrick. This is the full list of BioBricks we plan on using in our project:<br> | ||
+ | <table border="1"> | ||
+ | <tr> | ||
+ | <th>BioBrick</th> | ||
+ | <td>B0010<br> | ||
+ | 5.13 D<br> | ||
+ | pSB1C3<br> | ||
+ | Terminator</td> | ||
+ | <td>B0012 | ||
+ | 3.1A<br> | ||
+ | pSB1C3<br> | ||
+ | Terminator | ||
+ | </td> | ||
+ | <td>B0032<br> | ||
+ | 3.1I<br> | ||
+ | pSB1C3<br> | ||
+ | RBS | ||
+ | </td> | ||
+ | <td>B1009 | ||
+ | 3.6J<br> | ||
+ | pSB1C3<br> | ||
+ | Terminator<br> | ||
+ | </td> | ||
+ | <td>C0060<br> | ||
+ | 3.3G<br> | ||
+ | pSB1C3<br> | ||
+ | aiiA + LVA<br> | ||
+ | </td> | ||
+ | <td>C0061<br> | ||
+ | 3.3I<br> | ||
+ | pSB1C3<br> | ||
+ | luxI + LVA<br> | ||
+ | </td> | ||
+ | <td>C0062<br> | ||
+ | 3.3K<br> | ||
+ | pSB1C3<br> | ||
+ | luxR<br> | ||
+ | </td> | ||
+ | <td>C0070<br> | ||
+ | 3.8N<br> | ||
+ | pSB1C3<br> | ||
+ | rhII + LVA<br> | ||
+ | </td> | ||
+ | <td>C0071<br> | ||
+ | 3.8P<br> | ||
+ | pSB1C3<br> | ||
+ | rhIR + LVA<br> | ||
+ | </td> | ||
+ | |||
+ | |||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>DNA<br>concentration<br> [ng/µL]</th> | ||
+ | <td>91.4</td> | ||
+ | <td>56.7</td> | ||
+ | <td>53.4</td> | ||
+ | <td>48.4</td> | ||
+ | <td>50.2</td> | ||
+ | <td>61.8</td> | ||
+ | <td>62.2</td> | ||
+ | <td>52.7</td> | ||
+ | <td>50.0</td> | ||
+ | |||
+ | |||
+ | </tr> | ||
+ | </table> | ||
+ | <table border="1"> | ||
+ | <tr> | ||
+ | <th>BioBrick</th> | ||
+ | <td>C0078<br> | ||
+ | 3.10N<br> | ||
+ | pSB1C3<br> | ||
+ | lasI + LVA<br> | ||
+ | </td> | ||
+ | <td>C0079<br> | ||
+ | 3.10P<br> | ||
+ | pSB1C3<br> | ||
+ | lasR + LVA<br> | ||
+ | </td> | ||
+ | <td>E0240<br> | ||
+ | 3.23A<br> | ||
+ | pSB1C3<br> | ||
+ | GFP<br> | ||
+ | </td> | ||
+ | <td>E0430<br> | ||
+ | 3.20K<br> | ||
+ | pSB1C3<br> | ||
+ | YFP<br> | ||
+ | </td> | ||
+ | <td>J06702<br> | ||
+ | 3.16B<br> | ||
+ | pSB1C3<br> | ||
+ | RFP<br> | ||
+ | </td> | ||
+ | <td>J23106<br> | ||
+ | 5.18O<br> | ||
+ | BBa_J61002<br> | ||
+ | Pcon medium<br> | ||
+ | </td> | ||
+ | <td>J23100<br> | ||
+ | 5.18C<br> | ||
+ | BBa_J61002<br> | ||
+ | Pcon strong<br> | ||
+ | </td> | ||
+ | <td>J23112<br> | ||
+ | 5.20E<br> | ||
+ | BBa_J61002<br> | ||
+ | Pcon weak<br> | ||
+ | </td> | ||
+ | <td>R0062<br> | ||
+ | 3.5G<br> | ||
+ | pSB1C3<br> | ||
+ | pLux<br> | ||
+ | </td> | ||
+ | <td>R0071<br> | ||
+ | 3.6E<br> | ||
+ | pSB1C3<br> | ||
+ | pRhl<br> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>DNA<br>concentration<br>[ng/µL]</th> | ||
+ | <td>49.3</td> | ||
+ | <td>53.6</td> | ||
+ | <td>50.7</td> | ||
+ | <td>52.7</td> | ||
+ | <td>52.7</td> | ||
+ | <td>70.4</td> | ||
+ | <td>73.1</td> | ||
+ | <td>78.1</td> | ||
+ | <td>53.0</td> | ||
+ | <td>51.6</td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | |||
</p> | </p> | ||
Revision as of 23:30, 10 September 2013
Labjournal
This is the documentation of our lab work. For detailed protocols of certain procedures please refer to Protocols. Attributions are given for each day, however please check our Attributions section for efforts beyond the lab work.
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