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10/09/13
From 2013.igem.org
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[[File:doubledigest_TODX_10013.jpg]] | [[File:doubledigest_TODX_10013.jpg]] | ||
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| + | Wells order: | ||
| + | TODX1 (SpeI/XbaI), TODX1 (EcoRI/PstI); TODX2 (SpeI/XbaI), TODX2 (EcoRI/PstI); TODX3 (SpeI/XbaI), TODX3 (EcoRI/PstI); TODX4 (SpeI/XbaI), TODX4 (EcoRI/PstI); TODX5 (SpeI/XbaI), TODX5 (EcoRI/PstI); TODX6 (SpeI/XbaI), TODX6 (EcoRI/PstI); | ||
| + | |||
| + | The expected results were that the we would see the TODX genes drop off in separate band in all the digestions in their respective sizes. However | ||
Revision as of 13:45, 11 September 2013
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Dissolution of polystyrene
- Today a 20ml solution of 50% Limonene would be made to determine if the dissolution of polystyrene from 09/09/13 would occur faster.
Gel electrophoresis
- The samples from the double digest on 09/09/13 were run to confirm if the digestion worked.
- From the results, we would also be able to distinguish between cells that have been successfully transformed with the ligated pSB1C3/TOD genes from those with the recircularised pSB1C3.
Results of the Gel electrophoresis
TODX Gel
Wells order: TODX1 (SpeI/XbaI), TODX1 (EcoRI/PstI); TODX2 (SpeI/XbaI), TODX2 (EcoRI/PstI); TODX3 (SpeI/XbaI), TODX3 (EcoRI/PstI); TODX4 (SpeI/XbaI), TODX4 (EcoRI/PstI); TODX5 (SpeI/XbaI), TODX5 (EcoRI/PstI); TODX6 (SpeI/XbaI), TODX6 (EcoRI/PstI);
The expected results were that the we would see the TODX genes drop off in separate band in all the digestions in their respective sizes. However
