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12/09/13
From 2013.igem.org
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*Samples were then frozen at -80 | *Samples were then frozen at -80 | ||
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[[File:doubledigest_gelpurification_12092013.jpg]] | [[File:doubledigest_gelpurification_12092013.jpg]] | ||
Revision as of 09:19, 13 September 2013
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Isolate plasmid from overnight culture
- Using Omega mini prep kit protocol
- Samples:
- TODF 1,2,3,
- TODX 1, 2, 3
- TOBG 1, 2, 3,
- RFP 1, 2, 3
Glycerol stocks
- Took 750ul from overnight culture and centrifuged at full speed for ~2 minutes
- Supernatant was removed
- Pellet resuspended in 375ul of HMFM
- Samples were then frozen at -80
Double Digest
