12/09/13

From 2013.igem.org

(Difference between revisions)
(→Isolate plasmid from overnight culture)
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**RFP 1, 2, 3
**RFP 1, 2, 3
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{|border=1
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|-
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|sample||Volume (ul)||Concentration (ng/ul)
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|-
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|TODF1||43||120
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|-
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|TODF2||43||98.8
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|-
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|TODX1||42||399.3
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|-
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|TODX2||44||442.2
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|-
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|TODX3||43||417.5
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|-
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|TOBG1||43||262.4
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|-
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|TOBG2||47||319.2
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|-
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|TOBG3||41||338.4
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|-
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|RFP1||41||77.7
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|-
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|RFP2||41||32.7
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|-
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|RFP3||42||59
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|}
==Glycerol stocks==
==Glycerol stocks==

Revision as of 09:33, 13 September 2013

Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions


Isolate plasmid from overnight culture

  • Using Omega mini prep kit protocol
  • Samples:
    • TODF 1,2,3,
    • TODX 1, 2, 3
    • TOBG 1, 2, 3,
    • RFP 1, 2, 3
sampleVolume (ul)Concentration (ng/ul)
TODF143120
TODF24398.8
TODX142399.3
TODX244442.2
TODX343417.5
TOBG143262.4
TOBG247319.2
TOBG341338.4
RFP14177.7
RFP24132.7
RFP34259

Glycerol stocks

  • Took 750ul from overnight culture and centrifuged at full speed for ~2 minutes
  • Supernatant was removed
  • Pellet resuspended in 375ul of HMFM
  • Samples were then frozen at -80


Double Digest

Doubledigest gelpurification 12092013.jpg

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