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16/09/13
From 2013.igem.org
(Difference between revisions)
(→Digesting pSB1C3 backbone) |
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*Exluding the DpnI from the master mix and adjusting the volume of water accordingly | *Exluding the DpnI from the master mix and adjusting the volume of water accordingly | ||
*Protocol can be found at: [http://parts.igem.org/Help:Protocols/Linearized_Plasmid_Backbones] | *Protocol can be found at: [http://parts.igem.org/Help:Protocols/Linearized_Plasmid_Backbones] | ||
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| + | ==Digestion of the TOD genes PCR products== | ||
==Running the backbone and RFP on gel== | ==Running the backbone and RFP on gel== | ||
| - | *on 1% gel to check if | + | *on 1% gel to check if the gel extraction worked. |
Revision as of 12:13, 16 September 2013
Digesting pSB1C3 backbone
- Digesting for ligation with the already digested Tod operon PCR products
- Following the iGEM official protocol
- Using EcoRI-HF and PstI-HF
- Exluding the DpnI from the master mix and adjusting the volume of water accordingly
- Protocol can be found at: [http://parts.igem.org/Help:Protocols/Linearized_Plasmid_Backbones]
Digestion of the TOD genes PCR products
Running the backbone and RFP on gel
- on 1% gel to check if the gel extraction worked.
