User contributions

From 2013.igem.org

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• 15:43, 4 October 2013 (diff | hist) Team:Leicester/Human Practises ‎
• 15:41, 4 October 2013 (diff | hist) N Team:Leicester/Human Practises ‎ (Created page with "==Human Practises==")
• 15:39, 4 October 2013 (diff | hist) Team:Leicester/Attributions ‎
• 15:38, 4 October 2013 (diff | hist) Team:Leicester/Attributions ‎
• 15:37, 4 October 2013 (diff | hist) Team:Leicester/Safety ‎
• 15:37, 4 October 2013 (diff | hist) Team:Leicester/Notebook ‎
• 15:37, 4 October 2013 (diff | hist) Team:Leicester/Parts ‎
• 15:36, 4 October 2013 (diff | hist) Team:Leicester/Project ‎
• 15:36, 4 October 2013 (diff | hist) Team:Leicester/Team ‎ (top)
• 15:35, 4 October 2013 (diff | hist) Team:Leicester/Team ‎
• 15:34, 4 October 2013 (diff | hist) Team:Leicester/Modeling ‎ (top)
• 15:33, 4 October 2013 (diff | hist) Team:Leicester/Modeling ‎
• 15:29, 4 October 2013 (diff | hist) Team:Leicester/Modeling ‎
• 15:28, 4 October 2013 (diff | hist) Team:Leicester/Modeling ‎
• 15:27, 4 October 2013 (diff | hist) Team:Leicester/Modeling ‎
• 15:23, 4 October 2013 (diff | hist) Team:Leicester/Modeling ‎
• 15:21, 4 October 2013 (diff | hist) Team:Leicester/Modeling ‎
• 15:20, 4 October 2013 (diff | hist) Team:Leicester/Modeling ‎
• 15:18, 4 October 2013 (diff | hist) Team:Leicester ‎
• 15:17, 4 October 2013 (diff | hist) Team:Leicester/Modeling ‎
• 10:03, 20 September 2013 (diff | hist) N 20/09/13 ‎ (Created page with "==Miniprep from overnight broths== *DNA is isolated using Omega Miniprep kit and its protocol is followed ==Nanodropping the isolated DNA== {|border=1 |Sample||Volume ul||Concen...")
• 09:59, 20 September 2013 (diff | hist) Team:Leicester/Notebook ‎
• 09:59, 20 September 2013 (diff | hist) 18/09/13 ‎ (→Plating out TOD operon bacteria) (top)
• 09:58, 20 September 2013 (diff | hist) 19/09/13 ‎
• 09:57, 20 September 2013 (diff | hist) 19/09/13 ‎
• 10:45, 18 September 2013 (diff | hist) N 18/09/13 ‎ (Created page with "==Making and running an agarose gel== *Making a 3% agarose gel to run Tod PCR fusion genes from 17/09 *This is to determine that the digestion works *Running the gel with digeste...")
• 10:41, 18 September 2013 (diff | hist) 17/09/13 ‎ (→Ligation of Tod genes and pSB1C3 backbone)
• 10:37, 18 September 2013 (diff | hist) 17/09/13 ‎ (→Ligation of Tod genes and pSB1C3 backbone)
• 10:37, 18 September 2013 (diff | hist) 17/09/13 ‎ (→Ligation of Tod genes and pSB1C3 backbone)
• 10:37, 18 September 2013 (diff | hist) 17/09/13 ‎ (→Ligation of Tod genes and pSB1C3 backbone)
• 10:36, 18 September 2013 (diff | hist) 17/09/13 ‎ (→Digestion of new fusion PCR Tod genes)
• 10:23, 18 September 2013 (diff | hist) 17/09/13 ‎ (→Digestion of new fusion PCR Tod genes)
• 10:16, 18 September 2013 (diff | hist) 17/09/13 ‎ (→Results from previous day)
• 10:08, 18 September 2013 (diff | hist) N 17/09/13 ‎ (Created page with "==Results from previous day== *Background control plate had growth, which was not expected. This suggests that pSB1C3 backbone religated. *It was also thought that the Tod genes...")
• 10:02, 18 September 2013 (diff | hist) Team:Leicester/Notebook ‎
• 14:52, 16 September 2013 (diff | hist) 16/09/13 ‎ (→Digestion of the pGEM plasmid gel extracted on the 13/09/13)
• 14:52, 16 September 2013 (diff | hist) 16/09/13 ‎ (→Digestion of the pGEM plasmid extracted on the 13/09/13)
• 14:08, 16 September 2013 (diff | hist) 16/09/13 ‎ (→Running the backbone and RFP on gel)
• 14:05, 16 September 2013 (diff | hist) 16/09/13 ‎ (→Ligation of digested pSB1C3 backbone to Tod F, X and Tob G)
• 14:04, 16 September 2013 (diff | hist) 16/09/13 ‎ (→Transformation of TOD genes ligations done on the 02/09/13 using the pGEM-T Vector System I-A3600 (changes were made to the protocol))
• 14:02, 16 September 2013 (diff | hist) 16/09/13 ‎ (→Ligation of digested pSB1C3 backbone to Tod F, X and Tob G)
• 14:01, 16 September 2013 (diff | hist) 16/09/13 ‎ (→Ligation of digested pSB1C3 backbone to Tod F, X and Tob G)
• 14:01, 16 September 2013 (diff | hist) 16/09/13 ‎
• 12:30, 16 September 2013 (diff | hist) 16/09/13 ‎
• 12:27, 16 September 2013 (diff | hist) 16/09/13 ‎ (→Digestion of the TOD genes PCR products)
• 12:26, 16 September 2013 (diff | hist) 16/09/13 ‎ (→Digestion of the TOD genes PCR products)
• 12:18, 16 September 2013 (diff | hist) 16/09/13 ‎ (→Digestion of the TOD genes PCR products)
• 12:13, 16 September 2013 (diff | hist) 16/09/13 ‎
• 11:21, 16 September 2013 (diff | hist) 16/09/13 ‎ (→Digesting pSB1C3 backbone)
• 11:20, 16 September 2013 (diff | hist) N 16/09/13 ‎ (Created page with "==Digesting pSB1C3 backbone== *Digesting for ligation with the already digested Tod operon PCR products *Following the iGEM official protocol *Using EcoRI-HF and PstI-HF *Exludin...")

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