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13/09/13
From 2013.igem.org
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(→Gel electropheresis of pGEM-T vectors with the TOD genes) |
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==Gel electropheresis of pGEM-T vectors with the TOD genes== | ==Gel electropheresis of pGEM-T vectors with the TOD genes== | ||
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| + | [[File:Doubledigest_TODgenes_130913.jpg]] | ||
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| + | Gel order: TodX1, TodX2, TodX3, TodF1, TodF2, TobG1, TobG2, TobG3 (all cut with (XbaI/PstI); TodX1, TodX2, TodX3 (this were cut with EcoRI/SpeI) | ||
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| + | The gel shows that once again the expected genes were not cut out from the plasmid. This could be because the restriction sites were mutated when they were cloned. | ||
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Gel electropheresis of pGEM-T vectors with the TOD genes
Gel order: TodX1, TodX2, TodX3, TodF1, TodF2, TobG1, TobG2, TobG3 (all cut with (XbaI/PstI); TodX1, TodX2, TodX3 (this were cut with EcoRI/SpeI)
The gel shows that once again the expected genes were not cut out from the plasmid. This could be because the restriction sites were mutated when they were cloned.
