Team:DTU-Denmark/Notebook/26 July 2013

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=Lab 115=
=Lab 115=

Revision as of 20:43, 16 September 2013

26 July 2013

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Contents

Lab 115


Main purpose



Who was in the lab


Helen, Kasia

Procedure


Results


Conclusion


Lab 208


Main purpose


  • miniprep of TAT, Sec, and Nir for sequencing
  • LB medium preparation (2L)
  • Pick up new PAO1 cultures
  • Make new colony PCR to get Nir from the newly acquired colonies.

Who was in the lab


Kristian, Julia

Procedure


PCRs:

  • pZA21::RFP with primer pair 13 58C and 3:00.
  • araBAD from K808000 with primer pair 12 61C and 1:00
  • Colony PCR with first PAO1 colony but with a gradient of concentrations from 1x to 500x
  • Colony PCR with the 4 new plates of PAO1, concentration x1

Results


See tomorrows gel.

Conclusion


Nothing to conclude from today's work.

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