Team:DTU-Denmark/Notebook/27 July 2013

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Navigate to the [[Team:DTU-Denmark/Notebook/26_July_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/28_July_2013|Next]] Entry
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=lab ...=
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=Lab 208=
<hr/>
<hr/>
==Main purpose==
==Main purpose==
<hr/>
<hr/>
-
 
+
Run gel on yesterdays PCR.
==Who was in the lab==
==Who was in the lab==
<hr/>
<hr/>
-
 
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Kristian
==Procedure==
==Procedure==
<hr/>
<hr/>
 +
Gel casting and loading
==Results==
==Results==
<hr/>
<hr/>
 +
*1kb ladder
 +
*araBAD
 +
*araBAD
 +
*araBAD
 +
*Nir 1 min extension
 +
*Nir 1 min extension
 +
*pZA21::RFP no promoter
 +
*pZA21::RFP no promoter
 +
*pZA21::RFP no promoter
 +
*Negative control
 +
*Nir 1x
 +
*Nir 1x
 +
*Nir 5x
 +
*Nir 5x
 +
*1kb ladder
 +
 +
Next line:
 +
 +
*1kb ladder
 +
*Nir 10x
 +
*Nir 10x
 +
*Nir 50x
 +
*Nir 50x
 +
*Nir 100x
 +
*Nir 100x
 +
[[File:2013-07-26 ara Nir pZA21.jpg|600px]]
 +
 +
 +
*1kb ladder
 +
*Nir 500x
 +
*Nir 500x
 +
*Nir 2022
 +
*Nir 2022
 +
*Nir 2023
 +
*Nir 2023
 +
*Nir 2024
 +
*Nir 2024
 +
*Nir 2025
 +
*Nir 2025
 +
[[File:2013-07-26 Nir new colony.jpg|600px]]
 +
 +
==Conclusion==
==Conclusion==
<hr/>
<hr/>
 +
PCR is obviously not working too well for us right now. Tomorrow all reagents will be chanced and some of the PCR's will be run again.
 +
Navigate to the [[Team:DTU-Denmark/Notebook/26_July_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/28_July_2013|Next]] Entry
Navigate to the [[Team:DTU-Denmark/Notebook/26_July_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/28_July_2013|Next]] Entry
{{:Team:DTU-Denmark/Templates/EndPage}}
{{:Team:DTU-Denmark/Templates/EndPage}}

Latest revision as of 20:43, 16 September 2013

27 July 2013

Navigate to the Previous or the Next Entry

Contents

Lab 208

Main purpose

Run gel on yesterdays PCR.

Who was in the lab

Kristian

Procedure

Gel casting and loading

Results

  • 1kb ladder
  • araBAD
  • araBAD
  • araBAD
  • Nir 1 min extension
  • Nir 1 min extension
  • pZA21::RFP no promoter
  • pZA21::RFP no promoter
  • pZA21::RFP no promoter
  • Negative control
  • Nir 1x
  • Nir 1x
  • Nir 5x
  • Nir 5x
  • 1kb ladder

Next line:

  • 1kb ladder
  • Nir 10x
  • Nir 10x
  • Nir 50x
  • Nir 50x
  • Nir 100x
  • Nir 100x

2013-07-26 ara Nir pZA21.jpg


  • 1kb ladder
  • Nir 500x
  • Nir 500x
  • Nir 2022
  • Nir 2022
  • Nir 2023
  • Nir 2023
  • Nir 2024
  • Nir 2024
  • Nir 2025
  • Nir 2025

2013-07-26 Nir new colony.jpg


Conclusion

PCR is obviously not working too well for us right now. Tomorrow all reagents will be chanced and some of the PCR's will be run again.

Navigate to the Previous or the Next Entry

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