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Team:DTU-Denmark/Notebook/27 July 2013
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{{:Team:DTU-Denmark/Templates/StartPage|27 July 2013}} | {{:Team:DTU-Denmark/Templates/StartPage|27 July 2013}} | ||
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Navigate to the [[Team:DTU-Denmark/Notebook/26_July_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/28_July_2013|Next]] Entry | Navigate to the [[Team:DTU-Denmark/Notebook/26_July_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/28_July_2013|Next]] Entry | ||
=Lab 208= | =Lab 208= | ||
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==Procedure== | ==Procedure== | ||
<hr/> | <hr/> | ||
| + | Gel casting and loading | ||
==Results== | ==Results== | ||
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==Conclusion== | ==Conclusion== | ||
<hr/> | <hr/> | ||
| - | PCR is obviously not working | + | PCR is obviously not working too well for us right now. Tomorrow all reagents will be chanced and some of the PCR's will be run again. |
Navigate to the [[Team:DTU-Denmark/Notebook/26_July_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/28_July_2013|Next]] Entry | Navigate to the [[Team:DTU-Denmark/Notebook/26_July_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/28_July_2013|Next]] Entry | ||
{{:Team:DTU-Denmark/Templates/EndPage}} | {{:Team:DTU-Denmark/Templates/EndPage}} | ||
Latest revision as of 20:43, 16 September 2013
27 July 2013
Contents |
Lab 208
Main purpose
Run gel on yesterdays PCR.
Who was in the lab
Kristian
Procedure
Gel casting and loading
Results
- 1kb ladder
- araBAD
- araBAD
- araBAD
- Nir 1 min extension
- Nir 1 min extension
- pZA21::RFP no promoter
- pZA21::RFP no promoter
- pZA21::RFP no promoter
- Negative control
- Nir 1x
- Nir 1x
- Nir 5x
- Nir 5x
- 1kb ladder
Next line:
- 1kb ladder
- Nir 10x
- Nir 10x
- Nir 50x
- Nir 50x
- Nir 100x
- Nir 100x
- 1kb ladder
- Nir 500x
- Nir 500x
- Nir 2022
- Nir 2022
- Nir 2023
- Nir 2023
- Nir 2024
- Nir 2024
- Nir 2025
- Nir 2025
Conclusion
PCR is obviously not working too well for us right now. Tomorrow all reagents will be chanced and some of the PCR's will be run again.
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