Team:NCTU Formosa/results
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===RBS efficiency=== | ===RBS efficiency=== | ||
+ | We used the following biobrick to test the efficiency of different ribosome binding sites: | ||
+ | Pcons+'''B0034'''+mRFP+B0015 <br> | ||
+ | Pcons+'''Target 1'''+mRFP+J61048 <br> | ||
+ | Pcons+'''B0030'''+mRFP+B0015 <br> | ||
+ | Pcons+'''B0032'''+mRFP+B0015 <br> | ||
+ | control:pet40 | ||
+ | |||
+ | The control is pet40 and the reporter gene is mRFP, Figure 1 and Figure 2 show the difference of color. | ||
+ | |||
<span class="undetermined">(undetermined)</span> | <span class="undetermined">(undetermined)</span> | ||
[[File:Result_rbs_efficiency.jpg|center|600px|Fig 1. From left to right, the ribosome biding site respectively is: B0032, Target 1, B0030, B0034, control.]] | [[File:Result_rbs_efficiency.jpg|center|600px|Fig 1. From left to right, the ribosome biding site respectively is: B0032, Target 1, B0030, B0034, control.]] |
Revision as of 07:12, 20 September 2013
Results
The current progress of our project, including detailed information of the experimental data and the overall evaluation of the practicability of this project.
Contents |
RBS efficiency
We used the following biobrick to test the efficiency of different ribosome binding sites:
Pcons+B0034+mRFP+B0015
Pcons+Target 1+mRFP+J61048
Pcons+B0030+mRFP+B0015
Pcons+B0032+mRFP+B0015
control:pet40
The control is pet40 and the reporter gene is mRFP, Figure 1 and Figure 2 show the difference of color.
(undetermined)
Modeling
(undetermined)