Team:NCTU Formosa/results

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(→RBS efficiency)
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<p>We used the following biobrick to test the efficiency of different ribosome binding sites:
<p>We used the following biobrick to test the efficiency of different ribosome binding sites:
#Pcons+'''BBa_B0034'''+mRFP+Ter  
#Pcons+'''BBa_B0034'''+mRFP+Ter  
-
#Pcons+'''BBa_K1017202(rRBS-1)'''+mRFP+Ter  
+
#Pcons+'''[[BBa_K1017202]](rRBS-2)'''+mRFP+Ter  
#Pcons+'''BBa_B0030'''+mRFP+Ter  
#Pcons+'''BBa_B0030'''+mRFP+Ter  
#Pcons+'''BBa_B0032'''+mRFP+Ter  
#Pcons+'''BBa_B0032'''+mRFP+Ter  

Revision as of 07:26, 20 September 2013

Results

The current progress of our project, including detailed information of the experimental data and the overall evaluation of the practicability of this project.

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Contents

RBS efficiency

We used the following biobrick to test the efficiency of different ribosome binding sites:

  1. Pcons+BBa_B0034+mRFP+Ter
  2. Pcons+BBa_K1017202(rRBS-2)+mRFP+Ter
  3. Pcons+BBa_B0030+mRFP+Ter
  4. Pcons+BBa_B0032+mRFP+Ter
  5. control:pet40

The control is pet40 and the reporter gene is mRFP, Figure 1 and Figure 2 show the difference of color.

(undetermined)

Fig 1. From left to right, the ribosome biding site respectively is: B0032, Target 1, B0030, B0034, control.
Fig 2. From left to right, the ribosome biding site respectively is: B0034, Target 1, B0030, B0032, control.

Modeling

(undetermined)

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