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19/09/13
From 2013.igem.org
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==Sending isolated DNA to be sequenced by PNACL== | ==Sending isolated DNA to be sequenced by PNACL== | ||
Revision as of 09:58, 20 September 2013
Contents |
DNA isolation from PCR products
- Isolating DNA using Omega nucleic acid purification kit and following its protocol.
- Isolated DNA from all of the samples B and F and samples X3, 5, 6, 7, 8, 10, 12.
- Selection was made by looking at gel pictures and selecting samples with visible bands.
Nanodropping the samples for concentration
| Sample nr | Volume ul | Concentration ng/ul | 260/280 | 260/230 |
Sending isolated DNA to be sequenced by PNACL
- Sending 4 samples from each operon that showed highest DNA concentration: B5, 4, 9, 12; F8, 1, 10, 12; X3, 5, 6, 12.
Preparing overnight broths
- Preparing broths from individual colonie plates from previous day
- Selecting 4 colonies from each that showed highest DNA concentration from nanodrop: B5, 4, 9, 12; F8, 1, 10, 12; X3, 5, 6, 12.
