"
19/09/13
From 2013.igem.org
(Difference between revisions)
(→Nanodropping the samples for concentration) |
|||
| Line 22: | Line 22: | ||
|Sample nr||Volume ul||Concentration ng/ul||260/280||260/230 | |Sample nr||Volume ul||Concentration ng/ul||260/280||260/230 | ||
|- | |- | ||
| - | + | |X3||11.3||1.87||1.29 | |
| + | |- | ||
| + | |X5||12.2||1.78||1.22 | ||
| + | |- | ||
| + | |X6||12.9||1.79||1.22 | ||
| + | |- | ||
| + | |X7||8||1.71||0.96 | ||
| + | |- | ||
| + | |X8||10.5||1.88||1.2 | ||
|} | |} | ||
Revision as of 10:22, 20 September 2013
| Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
|---|
Contents |
DNA isolation from PCR products
- Isolating DNA using Omega nucleic acid purification kit and following its protocol.
- Isolated DNA from all of the samples B and F and samples X3, 5, 6, 7, 8, 10, 12.
- Selection was made by looking at gel pictures and selecting samples with visible bands.
Nanodropping the samples for concentration
| Sample nr | Volume ul | Concentration ng/ul | 260/280 | 260/230 |
| X3 | 11.3 | 1.87 | 1.29 | |
| X5 | 12.2 | 1.78 | 1.22 | |
| X6 | 12.9 | 1.79 | 1.22 | |
| X7 | 8 | 1.71 | 0.96 | |
| X8 | 10.5 | 1.88 | 1.2 |
Sending isolated DNA to be sequenced by PNACL
- Sending 4 samples from each operon that showed highest DNA concentration: B5, 4, 9, 12; F8, 1, 10, 12; X3, 5, 6, 12.
Preparing overnight broths
- Preparing broths from individual colonie plates from previous day
- Selecting 4 colonies from each that showed highest DNA concentration from nanodrop: B5, 4, 9, 12; F8, 1, 10, 12; X3, 5, 6, 12.
