"
Team:BYU Provo/Notebook/CholeraDetection/Springexp/Period2/Dailylog
From 2013.igem.org
(Difference between revisions)
| Line 4: | Line 4: | ||
{| width="100%" | {| width="100%" | ||
| - | | colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Cholera Detection | + | | colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Cholera Detection May-June Notebook: May 15 - May Daily Log'''</font> |
<br> | <br> | ||
| Line 31: | Line 31: | ||
<font face="Calibri" size="3"> | <font face="Calibri" size="3"> | ||
| - | <font size="4"> ''' | + | <font size="4"> '''5/15/13''' </font> |
On Saturday we checked our bacteriophage spot test for plaques, but we saw none. Our bacteriophage lambda purification procedure failed. Dr. Grose thinks that inducing lambda to lysis by 43 degree heat shock must not have been sufficient stress. Since we know H202 does induce our phage, we're going to attempt to isolate bacteriophage lambda by doing a top agar plaque assay with a drop of hydrogen peroxide, then bathing the top agar in LB, sucking it all up and then purifying the bacteriophage by chloroform lysis procedure. | On Saturday we checked our bacteriophage spot test for plaques, but we saw none. Our bacteriophage lambda purification procedure failed. Dr. Grose thinks that inducing lambda to lysis by 43 degree heat shock must not have been sufficient stress. Since we know H202 does induce our phage, we're going to attempt to isolate bacteriophage lambda by doing a top agar plaque assay with a drop of hydrogen peroxide, then bathing the top agar in LB, sucking it all up and then purifying the bacteriophage by chloroform lysis procedure. | ||
| Line 38: | Line 38: | ||
KK, KP, CH | KK, KP, CH | ||
| - | |||
| - | |||
| - | |||
| - | |||
<br> | <br> | ||
{{TeamBYUProvoFooter}} | {{TeamBYUProvoFooter}} | ||
Revision as of 04:05, 21 September 2013
| Cholera Detection May-June Notebook: May 15 - May Daily Log
| ||
|
|
5/15/13 On Saturday we checked our bacteriophage spot test for plaques, but we saw none. Our bacteriophage lambda purification procedure failed. Dr. Grose thinks that inducing lambda to lysis by 43 degree heat shock must not have been sufficient stress. Since we know H202 does induce our phage, we're going to attempt to isolate bacteriophage lambda by doing a top agar plaque assay with a drop of hydrogen peroxide, then bathing the top agar in LB, sucking it all up and then purifying the bacteriophage by chloroform lysis procedure. Clarice has been working on submitting parts to the registry. She has cloned the Ydiv gene into the iGEM backbone to submit the part; we purified the plasmid for submission. KK, KP, CH
|
|
