Team:Buenos Aires/ prueba

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Latest revision as of 02:28, 23 September 2013

Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012

Wetlab work at UBA labs

We chose for the chassis the cyanobacteria Synechocystis PCC 6803, because it is a photoautotroph and is regulated by circadian rhythms.

Using the lux operon and circadian promoters, we designed two plasmids: the Luciferase generator is to produce bioluminiscence, and the substrate generator recovers substainably the substrates necessary for the reactions.

We achieved to transform Synechocystis with the luciferase generator plasmid, and verificated light emission in a luminometer.

Al being lit by bioluminescent bacteria!

The Project

To Drink or Not To Drink
File:Howworks.gif

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